Experiment: Control_Nissle
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS00010 and ECOLIN_RS00015 are separated by 1 nucleotides ECOLIN_RS00015 and ECOLIN_RS00020 are separated by 0 nucleotides ECOLIN_RS00020 and ECOLIN_RS00030 are separated by 213 nucleotides ECOLIN_RS00030 and ECOLIN_RS00035 are separated by 59 nucleotides
ECOLIN_RS00010: ECOLIN_RS00010 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 361 to 2,823
_RS00010
ECOLIN_RS00015: ECOLIN_RS00015 - homoserine kinase, at 2,825 to 3,757
_RS00015
ECOLIN_RS00020: ECOLIN_RS00020 - threonine synthase, at 3,758 to 5,044
_RS00020
ECOLIN_RS00030: ECOLIN_RS00030 - DUF2502 domain-containing protein, at 5,258 to 5,554
_RS00030
ECOLIN_RS00035: ECOLIN_RS00035 - peroxide stress protein YaaA, at 5,614 to 6,390
_RS00035
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -1
0
1 at 2.796 kb on + strand at 2.928 kb on + strand, within ECOLIN_RS00015 at 3.021 kb on - strand, within ECOLIN_RS00015 at 3.021 kb on - strand, within ECOLIN_RS00015 at 3.467 kb on + strand, within ECOLIN_RS00015 at 4.270 kb on + strand, within ECOLIN_RS00020 at 4.270 kb on + strand, within ECOLIN_RS00020 at 4.270 kb on + strand, within ECOLIN_RS00020 at 4.271 kb on - strand, within ECOLIN_RS00020 at 4.271 kb on - strand, within ECOLIN_RS00020 at 4.565 kb on - strand, within ECOLIN_RS00020 at 4.740 kb on - strand, within ECOLIN_RS00020 at 4.824 kb on + strand, within ECOLIN_RS00020 at 5.276 kb on - strand at 5.439 kb on - strand, within ECOLIN_RS00030 at 5.439 kb on - strand, within ECOLIN_RS00030 at 5.439 kb on - strand, within ECOLIN_RS00030 at 5.494 kb on + strand, within ECOLIN_RS00030 at 5.783 kb on - strand, within ECOLIN_RS00035 at 5.888 kb on - strand, within ECOLIN_RS00035 at 5.894 kb on - strand, within ECOLIN_RS00035 at 5.944 kb on - strand, within ECOLIN_RS00035
Per-strain Table
Position Strand Gene LocusTag Fraction Control_Nissle remove 2,796 + -0.5 2,928 + ECOLIN_RS00015 0.11 -0.3 3,021 - ECOLIN_RS00015 0.21 -0.7 3,021 - ECOLIN_RS00015 0.21 +0.4 3,467 + ECOLIN_RS00015 0.69 +0.4 4,270 + ECOLIN_RS00020 0.40 +0.4 4,270 + ECOLIN_RS00020 0.40 -0.3 4,270 + ECOLIN_RS00020 0.40 -0.2 4,271 - ECOLIN_RS00020 0.40 +0.7 4,271 - ECOLIN_RS00020 0.40 +0.7 4,565 - ECOLIN_RS00020 0.63 +0.3 4,740 - ECOLIN_RS00020 0.76 -0.4 4,824 + ECOLIN_RS00020 0.83 +0.3 5,276 - +0.6 5,439 - ECOLIN_RS00030 0.61 +1.0 5,439 - ECOLIN_RS00030 0.61 -0.1 5,439 - ECOLIN_RS00030 0.61 +0.3 5,494 + ECOLIN_RS00030 0.79 -0.5 5,783 - ECOLIN_RS00035 0.22 -0.9 5,888 - ECOLIN_RS00035 0.35 +1.0 5,894 - ECOLIN_RS00035 0.36 +0.0 5,944 - ECOLIN_RS00035 0.42 -0.9
Or see this region's nucleotide sequence