Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03120

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03115 and ECOLIN_RS03120 are separated by 149 nucleotidesECOLIN_RS03120 and ECOLIN_RS03125 overlap by 11 nucleotidesECOLIN_RS03125 and ECOLIN_RS03130 are separated by 156 nucleotides ECOLIN_RS03115: ECOLIN_RS03115 - 'cyclic di-3',5'-guanylate-activated glycosyltransferase NrfB' pseudo=_no_value transl_table=11, at 639,777 to 642,014 _RS03115 ECOLIN_RS03120: ECOLIN_RS03120 - Cu(+)/Ag(+) sensor histidine kinase CusS, at 642,164 to 643,606 _RS03120 ECOLIN_RS03125: ECOLIN_RS03125 - copper response regulator transcription factor CusR, at 643,596 to 644,279 _RS03125 ECOLIN_RS03130: ECOLIN_RS03130 - Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC, at 644,436 to 645,818 _RS03130 Position (kb) 642 643 644Strain fitness (log2 ratio) -1 0 1at 642.026 kb on - strandat 642.702 kb on + strand, within ECOLIN_RS03120at 643.104 kb on + strand, within ECOLIN_RS03120at 643.154 kb on - strand, within ECOLIN_RS03120at 644.436 kb on - strandat 644.436 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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642,026 - +1.0
642,702 + ECOLIN_RS03120 0.37 -0.3
643,104 + ECOLIN_RS03120 0.65 +0.3
643,154 - ECOLIN_RS03120 0.69 +0.0
644,436 - +1.7
644,436 - -0.5

Or see this region's nucleotide sequence