Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS01095

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS01085 and ECOLIN_RS01090 are separated by 39 nucleotidesECOLIN_RS01090 and ECOLIN_RS01095 overlap by 8 nucleotidesECOLIN_RS01095 and ECOLIN_RS01100 are separated by 187 nucleotides ECOLIN_RS01085: ECOLIN_RS01085 - methionine ABC transporter substrate-binding lipoprotein MetQ, at 229,144 to 229,959 _RS01085 ECOLIN_RS01090: ECOLIN_RS01090 - methionine ABC transporter permease MetI, at 229,999 to 230,652 _RS01090 ECOLIN_RS01095: ECOLIN_RS01095 - methionine ABC transporter ATP-binding protein MetN, at 230,645 to 231,676 _RS01095 ECOLIN_RS01100: ECOLIN_RS01100 - D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase, at 231,864 to 232,436 _RS01100 Position (kb) 230 231 232Strain fitness (log2 ratio) -1 0 1at 230.111 kb on + strand, within ECOLIN_RS01090at 230.248 kb on - strand, within ECOLIN_RS01090at 230.344 kb on - strand, within ECOLIN_RS01090at 230.538 kb on - strand, within ECOLIN_RS01090at 230.673 kb on - strandat 230.789 kb on - strand, within ECOLIN_RS01095at 230.957 kb on - strand, within ECOLIN_RS01095at 231.003 kb on - strand, within ECOLIN_RS01095

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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230,111 + ECOLIN_RS01090 0.17 +0.2
230,248 - ECOLIN_RS01090 0.38 -0.1
230,344 - ECOLIN_RS01090 0.53 +0.1
230,538 - ECOLIN_RS01090 0.82 +0.1
230,673 - -0.2
230,789 - ECOLIN_RS01095 0.14 +0.9
230,957 - ECOLIN_RS01095 0.30 +0.6
231,003 - ECOLIN_RS01095 0.35 +0.2

Or see this region's nucleotide sequence