Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16620

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16610 and ECOLIN_RS32975 are separated by 145 nucleotidesECOLIN_RS32975 and ECOLIN_RS16620 are separated by 326 nucleotidesECOLIN_RS16620 and ECOLIN_RS16625 are separated by 3 nucleotidesECOLIN_RS16625 and ECOLIN_RS16635 are separated by 290 nucleotides ECOLIN_RS16610: ECOLIN_RS16610 - DUF417 family protein, at 3,356,154 to 3,356,582 _RS16610 ECOLIN_RS32975: ECOLIN_RS32975 - helicase, at 3,356,728 to 3,356,808 _RS32975 ECOLIN_RS16620: ECOLIN_RS16620 - hypothetical protein, at 3,357,135 to 3,357,350 _RS16620 ECOLIN_RS16625: ECOLIN_RS16625 - hypothetical protein, at 3,357,354 to 3,357,722 _RS16625 ECOLIN_RS16635: ECOLIN_RS16635 - hypothetical protein, at 3,358,013 to 3,358,255 _RS16635 Position (kb) 3357 3358Strain fitness (log2 ratio) -1 0 1at 3356.452 kb on + strand, within ECOLIN_RS16610at 3356.611 kb on + strandat 3356.765 kb on - strand, within ECOLIN_RS32975at 3356.952 kb on + strandat 3357.102 kb on + strandat 3357.103 kb on - strandat 3357.103 kb on - strandat 3357.114 kb on + strandat 3357.115 kb on - strandat 3357.115 kb on - strandat 3357.115 kb on - strandat 3357.219 kb on - strand, within ECOLIN_RS16620at 3357.315 kb on - strand, within ECOLIN_RS16620at 3357.380 kb on - strandat 3357.482 kb on + strand, within ECOLIN_RS16625at 3357.483 kb on - strand, within ECOLIN_RS16625at 3357.483 kb on - strand, within ECOLIN_RS16625at 3357.491 kb on + strand, within ECOLIN_RS16625at 3357.492 kb on - strand, within ECOLIN_RS16625at 3357.627 kb on - strand, within ECOLIN_RS16625at 3357.817 kb on - strandat 3357.887 kb on - strandat 3358.177 kb on + strand, within ECOLIN_RS16635at 3358.178 kb on - strand, within ECOLIN_RS16635at 3358.178 kb on - strand, within ECOLIN_RS16635at 3358.193 kb on - strand, within ECOLIN_RS16635at 3358.241 kb on + strandat 3358.242 kb on - strandat 3358.256 kb on + strandat 3358.256 kb on + strandat 3358.257 kb on - strandat 3358.257 kb on - strandat 3358.277 kb on - strandat 3358.277 kb on - strandat 3358.296 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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3,356,452 + ECOLIN_RS16610 0.69 -0.5
3,356,611 + -0.8
3,356,765 - ECOLIN_RS32975 0.46 +1.3
3,356,952 + -0.0
3,357,102 + +0.4
3,357,103 - -0.2
3,357,103 - +0.6
3,357,114 + -0.4
3,357,115 - +0.7
3,357,115 - +0.2
3,357,115 - +0.1
3,357,219 - ECOLIN_RS16620 0.39 +0.5
3,357,315 - ECOLIN_RS16620 0.83 -0.0
3,357,380 - +0.1
3,357,482 + ECOLIN_RS16625 0.35 -0.1
3,357,483 - ECOLIN_RS16625 0.35 +0.5
3,357,483 - ECOLIN_RS16625 0.35 +0.2
3,357,491 + ECOLIN_RS16625 0.37 +0.7
3,357,492 - ECOLIN_RS16625 0.37 +0.2
3,357,627 - ECOLIN_RS16625 0.74 -0.2
3,357,817 - +0.2
3,357,887 - +0.1
3,358,177 + ECOLIN_RS16635 0.67 +0.5
3,358,178 - ECOLIN_RS16635 0.68 +0.0
3,358,178 - ECOLIN_RS16635 0.68 +0.3
3,358,193 - ECOLIN_RS16635 0.74 -0.1
3,358,241 + -0.6
3,358,242 - -0.4
3,358,256 + -0.3
3,358,256 + -0.4
3,358,257 - -0.0
3,358,257 - +0.7
3,358,277 - +0.0
3,358,277 - -0.7
3,358,296 - -0.0

Or see this region's nucleotide sequence