Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04400

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04390 and ECOLIN_RS04395 are separated by 75 nucleotidesECOLIN_RS04395 and ECOLIN_RS04400 overlap by 1 nucleotidesECOLIN_RS04400 and ECOLIN_RS04405 are separated by 203 nucleotides ECOLIN_RS04390: ECOLIN_RS04390 - fructose-6-phosphate aldolase, at 889,646 to 890,308 _RS04390 ECOLIN_RS04395: ECOLIN_RS04395 - molybdopterin-synthase adenylyltransferase MoeB, at 890,384 to 891,133 _RS04395 ECOLIN_RS04400: ECOLIN_RS04400 - molybdopterin molybdotransferase MoeA, at 891,133 to 892,368 _RS04400 ECOLIN_RS04405: ECOLIN_RS04405 - beta-aspartyl-peptidase, at 892,572 to 893,537 _RS04405 Position (kb) 891 892 893Strain fitness (log2 ratio) -1 0 1at 890.457 kb on + strandat 890.458 kb on - strandat 890.458 kb on - strandat 890.458 kb on - strandat 890.728 kb on + strand, within ECOLIN_RS04395at 890.733 kb on - strand, within ECOLIN_RS04395at 891.246 kb on + strandat 891.251 kb on - strandat 891.252 kb on + strandat 891.252 kb on + strandat 891.495 kb on + strand, within ECOLIN_RS04400at 892.571 kb on + strandat 893.299 kb on - strand, within ECOLIN_RS04405at 893.332 kb on + strand, within ECOLIN_RS04405

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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890,457 + +0.5
890,458 - -0.9
890,458 - -0.3
890,458 - -1.5
890,728 + ECOLIN_RS04395 0.46 -1.2
890,733 - ECOLIN_RS04395 0.47 -0.2
891,246 + -0.1
891,251 - -0.0
891,252 + -0.2
891,252 + +0.1
891,495 + ECOLIN_RS04400 0.29 -0.4
892,571 + -0.4
893,299 - ECOLIN_RS04405 0.75 +0.9
893,332 + ECOLIN_RS04405 0.79 -0.4

Or see this region's nucleotide sequence