Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20090

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20085 and ECOLIN_RS20090 are separated by 798 nucleotidesECOLIN_RS20090 and ECOLIN_RS20095 overlap by 51 nucleotidesECOLIN_RS20095 and ECOLIN_RS20100 are separated by 107 nucleotides ECOLIN_RS20085: ECOLIN_RS20085 - acid-resistance protein HdeD, at 4,029,073 to 4,029,645 _RS20085 ECOLIN_RS20090: ECOLIN_RS20090 - acid resistance transcriptional activator GadE, at 4,030,444 to 4,030,971 _RS20090 ECOLIN_RS20095: ECOLIN_RS20095 - hypothetical protein, at 4,030,921 to 4,031,250 _RS20095 ECOLIN_RS20100: ECOLIN_RS20100 - multidrug transporter subunit MdtE, at 4,031,358 to 4,032,467 _RS20100 Position (kb) 4030 4031Strain fitness (log2 ratio) -1 0 1 2at 4029.591 kb on - strandat 4029.654 kb on - strandat 4029.867 kb on - strandat 4029.910 kb on - strandat 4029.933 kb on - strandat 4029.940 kb on + strandat 4029.975 kb on + strandat 4030.185 kb on + strandat 4030.185 kb on + strandat 4030.185 kb on + strandat 4030.257 kb on + strandat 4030.258 kb on - strandat 4030.258 kb on - strandat 4030.258 kb on - strandat 4030.271 kb on - strandat 4030.308 kb on + strandat 4030.314 kb on + strandat 4030.323 kb on + strandat 4030.323 kb on + strandat 4030.326 kb on + strandat 4030.391 kb on + strandat 4030.784 kb on + strand, within ECOLIN_RS20090at 4031.048 kb on - strand, within ECOLIN_RS20095at 4031.085 kb on - strand, within ECOLIN_RS20095at 4031.085 kb on - strand, within ECOLIN_RS20095at 4031.100 kb on + strand, within ECOLIN_RS20095at 4031.100 kb on + strand, within ECOLIN_RS20095at 4031.250 kb on + strandat 4031.251 kb on - strandat 4031.501 kb on - strand, within ECOLIN_RS20100at 4031.577 kb on + strand, within ECOLIN_RS20100at 4031.599 kb on + strand, within ECOLIN_RS20100at 4031.706 kb on + strand, within ECOLIN_RS20100at 4031.914 kb on + strand, within ECOLIN_RS20100at 4031.934 kb on + strand, within ECOLIN_RS20100at 4031.935 kb on - strand, within ECOLIN_RS20100at 4031.935 kb on - strand, within ECOLIN_RS20100

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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4,029,591 - +1.5
4,029,654 - -0.2
4,029,867 - +0.2
4,029,910 - -0.0
4,029,933 - -0.2
4,029,940 + +0.8
4,029,975 + +0.2
4,030,185 + -0.1
4,030,185 + +0.1
4,030,185 + -0.4
4,030,257 + -0.3
4,030,258 - -0.5
4,030,258 - +0.5
4,030,258 - +0.1
4,030,271 - -0.5
4,030,308 + +0.3
4,030,314 + +0.3
4,030,323 + +0.8
4,030,323 + +0.4
4,030,326 + +0.7
4,030,391 + -1.0
4,030,784 + ECOLIN_RS20090 0.64 -0.4
4,031,048 - ECOLIN_RS20095 0.38 -0.3
4,031,085 - ECOLIN_RS20095 0.50 -0.4
4,031,085 - ECOLIN_RS20095 0.50 +2.2
4,031,100 + ECOLIN_RS20095 0.54 +0.2
4,031,100 + ECOLIN_RS20095 0.54 +0.2
4,031,250 + +0.2
4,031,251 - +0.1
4,031,501 - ECOLIN_RS20100 0.13 -0.2
4,031,577 + ECOLIN_RS20100 0.20 -0.1
4,031,599 + ECOLIN_RS20100 0.22 -0.6
4,031,706 + ECOLIN_RS20100 0.31 +0.1
4,031,914 + ECOLIN_RS20100 0.50 +0.4
4,031,934 + ECOLIN_RS20100 0.52 -0.5
4,031,935 - ECOLIN_RS20100 0.52 -0.4
4,031,935 - ECOLIN_RS20100 0.52 +1.7

Or see this region's nucleotide sequence