Experiment: Bas63
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS14885 and ECOLIN_RS14890 are separated by 36 nucleotides ECOLIN_RS14890 and ECOLIN_RS14895 are separated by 151 nucleotides ECOLIN_RS14895 and ECOLIN_RS14900 are separated by 56 nucleotides
ECOLIN_RS14885: ECOLIN_RS14885 - L-alanine exporter AlaE, at 3,005,505 to 3,005,954
_RS14885
ECOLIN_RS14890: ECOLIN_RS14890 - DUF2002 family protein, at 3,005,991 to 3,006,335
_RS14890
ECOLIN_RS14895: ECOLIN_RS14895 - DUF883 domain-containing protein, at 3,006,487 to 3,006,816
_RS14895
ECOLIN_RS14900: ECOLIN_RS14900 - PLP-dependent aminotransferase family protein, at 3,006,873 to 3,008,207
_RS14900
Position (kb)
3006
3007 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3005.665 kb on + strand, within ECOLIN_RS14885 at 3005.766 kb on + strand, within ECOLIN_RS14885 at 3005.790 kb on + strand, within ECOLIN_RS14885 at 3005.892 kb on + strand, within ECOLIN_RS14885 at 3006.233 kb on - strand, within ECOLIN_RS14890 at 3006.331 kb on + strand at 3006.352 kb on + strand at 3006.353 kb on - strand at 3006.449 kb on + strand at 3006.880 kb on - strand at 3006.887 kb on + strand at 3007.421 kb on - strand, within ECOLIN_RS14900 at 3007.449 kb on - strand, within ECOLIN_RS14900 at 3007.714 kb on - strand, within ECOLIN_RS14900
Per-strain Table
Position Strand Gene LocusTag Fraction Bas63 remove 3,005,665 + ECOLIN_RS14885 0.36 -0.6 3,005,766 + ECOLIN_RS14885 0.58 -2.4 3,005,790 + ECOLIN_RS14885 0.63 +0.5 3,005,892 + ECOLIN_RS14885 0.86 +0.2 3,006,233 - ECOLIN_RS14890 0.70 -0.2 3,006,331 + +0.3 3,006,352 + +0.3 3,006,353 - +0.4 3,006,449 + -0.2 3,006,880 - +0.7 3,006,887 + +4.2 3,007,421 - ECOLIN_RS14900 0.41 +0.3 3,007,449 - ECOLIN_RS14900 0.43 +1.2 3,007,714 - ECOLIN_RS14900 0.63 +0.3
Or see this region's nucleotide sequence