Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13760

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13755 and ECOLIN_RS13760 are separated by 335 nucleotidesECOLIN_RS13760 and ECOLIN_RS13765 are separated by 48 nucleotides ECOLIN_RS13755: ECOLIN_RS13755 - Nramp family divalent metal transporter, at 2,765,919 to 2,767,157 _RS13755 ECOLIN_RS13760: ECOLIN_RS13760 - nucleoside permease NupC, at 2,767,493 to 2,768,695 _RS13760 ECOLIN_RS13765: ECOLIN_RS13765 - sensor domain-containing phosphodiesterase, at 2,768,744 to 2,770,933 _RS13765 Position (kb) 2767 2768 2769Strain fitness (log2 ratio) -1 0 1at 2767.003 kb on - strand, within ECOLIN_RS13755at 2767.003 kb on - strand, within ECOLIN_RS13755at 2767.064 kb on - strandat 2767.449 kb on - strandat 2767.987 kb on + strand, within ECOLIN_RS13760at 2767.987 kb on + strand, within ECOLIN_RS13760at 2768.045 kb on + strand, within ECOLIN_RS13760at 2768.047 kb on + strand, within ECOLIN_RS13760at 2768.048 kb on - strand, within ECOLIN_RS13760at 2768.069 kb on + strand, within ECOLIN_RS13760at 2768.216 kb on + strand, within ECOLIN_RS13760at 2768.701 kb on + strandat 2768.701 kb on + strandat 2768.701 kb on + strandat 2768.775 kb on - strandat 2769.041 kb on - strand, within ECOLIN_RS13765

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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2,767,003 - ECOLIN_RS13755 0.87 +0.5
2,767,003 - ECOLIN_RS13755 0.87 -1.3
2,767,064 - +0.3
2,767,449 - -0.8
2,767,987 + ECOLIN_RS13760 0.41 +1.5
2,767,987 + ECOLIN_RS13760 0.41 -1.6
2,768,045 + ECOLIN_RS13760 0.46 -0.3
2,768,047 + ECOLIN_RS13760 0.46 -0.8
2,768,048 - ECOLIN_RS13760 0.46 +0.1
2,768,069 + ECOLIN_RS13760 0.48 -0.8
2,768,216 + ECOLIN_RS13760 0.60 -0.6
2,768,701 + -1.3
2,768,701 + +0.3
2,768,701 + +0.5
2,768,775 - +0.0
2,769,041 - ECOLIN_RS13765 0.14 +0.4

Or see this region's nucleotide sequence