Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04815

Experiment: Bas63

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04810 and ECOLIN_RS04815 are separated by 169 nucleotidesECOLIN_RS04815 and ECOLIN_RS04820 are separated by 172 nucleotidesECOLIN_RS04820 and ECOLIN_RS04825 are separated by 110 nucleotides ECOLIN_RS04810: ECOLIN_RS04810 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 982,448 to 983,731 _RS04810 ECOLIN_RS04815: ECOLIN_RS04815 - metallopeptidase YcaL, at 983,901 to 984,665 _RS04815 ECOLIN_RS04820: ECOLIN_RS04820 - (d)CMP kinase, at 984,838 to 985,521 _RS04820 ECOLIN_RS04825: ECOLIN_RS04825 - 30S ribosomal protein S1, at 985,632 to 987,305 _RS04825 Position (kb) 983 984 985Strain fitness (log2 ratio) -1 0 1at 983.227 kb on - strand, within ECOLIN_RS04810at 983.368 kb on + strand, within ECOLIN_RS04810at 984.192 kb on + strand, within ECOLIN_RS04815at 984.663 kb on + strandat 984.664 kb on - strandat 984.708 kb on - strandat 984.788 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas63
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983,227 - ECOLIN_RS04810 0.61 +0.9
983,368 + ECOLIN_RS04810 0.72 -1.3
984,192 + ECOLIN_RS04815 0.38 +0.4
984,663 + -0.3
984,664 - +0.1
984,708 - -0.1
984,788 + +0.0

Or see this region's nucleotide sequence