Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS15970

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS15965 and ECOLIN_RS15970 are separated by 316 nucleotidesECOLIN_RS15970 and ECOLIN_RS15975 are separated by 78 nucleotides ECOLIN_RS15965: ECOLIN_RS15965 - amino acid permease, at 3,230,172 to 3,231,401 _RS15965 ECOLIN_RS15970: ECOLIN_RS15970 - tRNA-Gly, at 3,231,718 to 3,231,791 _RS15970 ECOLIN_RS15975: ECOLIN_RS15975 - amidase activator ActS, at 3,231,870 to 3,232,625 _RS15975 Position (kb) 3231 3232Strain fitness (log2 ratio) -1 0 1 2 3at 3231.200 kb on + strand, within ECOLIN_RS15965at 3231.750 kb on - strand, within ECOLIN_RS15970at 3231.784 kb on - strandat 3231.784 kb on - strandat 3231.905 kb on - strandat 3231.910 kb on - strandat 3232.123 kb on + strand, within ECOLIN_RS15975at 3232.124 kb on - strand, within ECOLIN_RS15975at 3232.343 kb on + strand, within ECOLIN_RS15975at 3232.344 kb on - strand, within ECOLIN_RS15975at 3232.344 kb on - strand, within ECOLIN_RS15975at 3232.406 kb on - strand, within ECOLIN_RS15975at 3232.477 kb on - strand, within ECOLIN_RS15975at 3232.477 kb on - strand, within ECOLIN_RS15975at 3232.711 kb on + strandat 3232.751 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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3,231,200 + ECOLIN_RS15965 0.84 +0.2
3,231,750 - ECOLIN_RS15970 0.43 -0.3
3,231,784 - -0.4
3,231,784 - +0.0
3,231,905 - +0.2
3,231,910 - +0.2
3,232,123 + ECOLIN_RS15975 0.33 +0.1
3,232,124 - ECOLIN_RS15975 0.34 +3.2
3,232,343 + ECOLIN_RS15975 0.63 -0.5
3,232,344 - ECOLIN_RS15975 0.63 +0.3
3,232,344 - ECOLIN_RS15975 0.63 +1.0
3,232,406 - ECOLIN_RS15975 0.71 -0.2
3,232,477 - ECOLIN_RS15975 0.80 +1.6
3,232,477 - ECOLIN_RS15975 0.80 +0.4
3,232,711 + -0.4
3,232,751 - +0.1

Or see this region's nucleotide sequence