Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS01685

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS27665 and ECOLIN_RS01685 are separated by 52 nucleotidesECOLIN_RS01685 and ECOLIN_RS01690 are separated by 24 nucleotides ECOLIN_RS27665: ECOLIN_RS27665 - PapB/FocB family fimbrial expression transcriptional regulator, at 335,885 to 336,187 _RS27665 ECOLIN_RS01685: ECOLIN_RS01685 - TolC family protein, at 336,240 to 337,487 _RS01685 ECOLIN_RS01690: ECOLIN_RS01690 - type I secretion system permease/ATPase, at 337,512 to 339,659 _RS01690 Position (kb) 336 337 338Strain fitness (log2 ratio) -1 0 1at 335.365 kb on - strandat 335.611 kb on - strandat 336.821 kb on + strand, within ECOLIN_RS01685at 337.075 kb on + strand, within ECOLIN_RS01685at 337.119 kb on + strand, within ECOLIN_RS01685at 337.336 kb on + strand, within ECOLIN_RS01685at 337.509 kb on + strandat 337.567 kb on + strandat 338.035 kb on + strand, within ECOLIN_RS01690at 338.046 kb on + strand, within ECOLIN_RS01690at 338.063 kb on + strand, within ECOLIN_RS01690at 338.206 kb on + strand, within ECOLIN_RS01690at 338.206 kb on + strand, within ECOLIN_RS01690

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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335,365 - +1.4
335,611 - +0.1
336,821 + ECOLIN_RS01685 0.47 +0.1
337,075 + ECOLIN_RS01685 0.67 +0.5
337,119 + ECOLIN_RS01685 0.70 +0.4
337,336 + ECOLIN_RS01685 0.88 +0.0
337,509 + -0.0
337,567 + -0.2
338,035 + ECOLIN_RS01690 0.24 +1.2
338,046 + ECOLIN_RS01690 0.25 -0.1
338,063 + ECOLIN_RS01690 0.26 +0.7
338,206 + ECOLIN_RS01690 0.32 -0.4
338,206 + ECOLIN_RS01690 0.32 -0.6

Or see this region's nucleotide sequence