Experiment: Bas52
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS30360 and ECOLIN_RS32140 are separated by 216 nucleotides ECOLIN_RS32140 and ECOLIN_RS32255 are separated by 48 nucleotides ECOLIN_RS32255 and ECOLIN_RS01880 are separated by 262 nucleotides ECOLIN_RS01880 and ECOLIN_RS27700 are separated by 70 nucleotides
ECOLIN_RS30360: ECOLIN_RS30360 - IS1 family transposase, at 375,073 to 375,766
_RS30360
ECOLIN_RS32140: ECOLIN_RS32140 - integrase, at 375,983 to 376,141
_RS32140
ECOLIN_RS32255: ECOLIN_RS32255 - hypothetical protein, at 376,190 to 376,327
_RS32255
ECOLIN_RS01880: ECOLIN_RS01880 - YagU family protein, at 376,590 to 377,204
_RS01880
ECOLIN_RS27700: ECOLIN_RS27700 - tail fiber assembly protein, at 377,275 to 377,441
_RS27700
Position (kb) 376
377 Strain fitness (log2 ratio) -1
0
1 at 375.196 kb on + strand, within ECOLIN_RS30360 at 375.643 kb on + strand, within ECOLIN_RS30360 at 376.055 kb on + strand, within ECOLIN_RS32140 at 376.562 kb on - strand at 376.597 kb on + strand at 376.886 kb on + strand, within ECOLIN_RS01880 at 376.957 kb on + strand, within ECOLIN_RS01880 at 377.198 kb on + strand at 377.232 kb on + strand at 377.254 kb on - strand at 377.306 kb on + strand, within ECOLIN_RS27700
Per-strain Table
Position Strand Gene LocusTag Fraction Bas52 remove 375,196 + ECOLIN_RS30360 0.18 +0.9 375,643 + ECOLIN_RS30360 0.82 +0.4 376,055 + ECOLIN_RS32140 0.45 -0.2 376,562 - +1.7 376,597 + +1.0 376,886 + ECOLIN_RS01880 0.48 -0.1 376,957 + ECOLIN_RS01880 0.60 -0.6 377,198 + -0.1 377,232 + -0.7 377,254 - -0.5 377,306 + ECOLIN_RS27700 0.19 -0.9
Or see this region's nucleotide sequence