Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS05225

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS05220 and ECOLIN_RS32690 are separated by 152 nucleotidesECOLIN_RS32690 and ECOLIN_RS05225 are separated by 280 nucleotidesECOLIN_RS05225 and ECOLIN_RS05230 are separated by 37 nucleotidesECOLIN_RS05230 and ECOLIN_RS05235 are separated by 20 nucleotides ECOLIN_RS05220: ECOLIN_RS05220 - curved DNA-binding protein, at 1,070,475 to 1,071,395 _RS05220 ECOLIN_RS32690: ECOLIN_RS32690 - hypothetical protein, at 1,071,548 to 1,071,649 _RS32690 ECOLIN_RS05225: ECOLIN_RS05225 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 1,071,930 to 1,073,172 _RS05225 ECOLIN_RS05230: ECOLIN_RS05230 - YccJ family protein, at 1,073,210 to 1,073,437 _RS05230 ECOLIN_RS05235: ECOLIN_RS05235 - NAD(P)H:quinone oxidoreductase, at 1,073,458 to 1,074,054 _RS05235 Position (kb) 1071 1072 1073 1074Strain fitness (log2 ratio) -1 0 1at 1071.228 kb on + strand, within ECOLIN_RS05220at 1071.228 kb on + strand, within ECOLIN_RS05220at 1071.302 kb on - strand, within ECOLIN_RS05220at 1071.445 kb on - strandat 1071.454 kb on - strandat 1071.554 kb on - strandat 1071.679 kb on + strandat 1072.183 kb on - strand, within ECOLIN_RS05225at 1072.647 kb on + strand, within ECOLIN_RS05225at 1073.041 kb on - strand, within ECOLIN_RS05225at 1073.270 kb on - strand, within ECOLIN_RS05230at 1073.270 kb on - strand, within ECOLIN_RS05230at 1073.381 kb on - strand, within ECOLIN_RS05230at 1073.493 kb on - strandat 1073.747 kb on - strand, within ECOLIN_RS05235

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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1,071,228 + ECOLIN_RS05220 0.82 -0.5
1,071,228 + ECOLIN_RS05220 0.82 +0.1
1,071,302 - ECOLIN_RS05220 0.90 -0.3
1,071,445 - -0.7
1,071,454 - +0.2
1,071,554 - -0.6
1,071,679 + -0.1
1,072,183 - ECOLIN_RS05225 0.20 +1.1
1,072,647 + ECOLIN_RS05225 0.58 +0.8
1,073,041 - ECOLIN_RS05225 0.89 +0.7
1,073,270 - ECOLIN_RS05230 0.26 +0.1
1,073,270 - ECOLIN_RS05230 0.26 -0.6
1,073,381 - ECOLIN_RS05230 0.75 +0.8
1,073,493 - -0.8
1,073,747 - ECOLIN_RS05235 0.48 +0.3

Or see this region's nucleotide sequence