Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS08145

Experiment: Bas52

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS08115 and ECOLIN_RS08120 overlap by 4 nucleotidesECOLIN_RS08120 and ECOLIN_RS08125 are separated by 7 nucleotidesECOLIN_RS08125 and ECOLIN_RS08145 are separated by 374 nucleotidesECOLIN_RS08145 and ECOLIN_RS08150 are separated by 218 nucleotides ECOLIN_RS08115: ECOLIN_RS08115 - YdbH family protein, at 1,586,280 to 1,588,919 _RS08115 ECOLIN_RS08120: ECOLIN_RS08120 - YnbE family lipoprotein, at 1,588,916 to 1,589,101 _RS08120 ECOLIN_RS08125: ECOLIN_RS08125 - YdbL family protein, at 1,589,109 to 1,589,435 _RS08125 ECOLIN_RS08145: ECOLIN_RS08145 - EntS/YbdA MFS transporter, at 1,589,810 to 1,598,411 _RS08145 ECOLIN_RS08150: ECOLIN_RS08150 - pyridoxine 4-dehydrogenase, at 1,598,630 to 1,599,490 _RS08150 Position (kb) 1589 1590 1591 1592 1593 1594 1595 1596 1597 1598 1599Strain fitness (log2 ratio) -1 0 1at 1590.446 kb on - strandat 1594.374 kb on - strand, within ECOLIN_RS08145at 1595.135 kb on + strand, within ECOLIN_RS08145at 1596.464 kb on + strand, within ECOLIN_RS08145at 1596.972 kb on + strand, within ECOLIN_RS08145at 1597.411 kb on + strand, within ECOLIN_RS08145at 1599.245 kb on + strand, within ECOLIN_RS08150

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas52
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1,590,446 - +1.3
1,594,374 - ECOLIN_RS08145 0.53 -1.2
1,595,135 + ECOLIN_RS08145 0.62 -1.1
1,596,464 + ECOLIN_RS08145 0.77 -0.6
1,596,972 + ECOLIN_RS08145 0.83 +0.6
1,597,411 + ECOLIN_RS08145 0.88 -0.8
1,599,245 + ECOLIN_RS08150 0.71 -0.7

Or see this region's nucleotide sequence