Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS23170

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS23160 and ECOLIN_RS23165 are separated by 59 nucleotidesECOLIN_RS23165 and ECOLIN_RS23170 are separated by 42 nucleotidesECOLIN_RS23170 and ECOLIN_RS23175 are separated by 170 nucleotides ECOLIN_RS23160: ECOLIN_RS23160 - methionine synthase, at 4,659,756 to 4,663,439 _RS23160 ECOLIN_RS23165: ECOLIN_RS23165 - hypothetical protein, at 4,663,499 to 4,663,819 _RS23165 ECOLIN_RS23170: ECOLIN_RS23170 - hypothetical protein, at 4,663,862 to 4,664,311 _RS23170 ECOLIN_RS23175: ECOLIN_RS23175 - Na/Pi cotransporter family protein, at 4,664,482 to 4,666,113 _RS23175 Position (kb) 4663 4664 4665Strain fitness (log2 ratio) -1 0 1at 4663.060 kb on - strand, within ECOLIN_RS23160at 4663.079 kb on + strandat 4663.087 kb on - strandat 4663.297 kb on - strandat 4663.320 kb on + strandat 4663.363 kb on - strandat 4663.681 kb on + strand, within ECOLIN_RS23165at 4664.248 kb on + strand, within ECOLIN_RS23170at 4664.375 kb on - strandat 4664.539 kb on - strandat 4664.664 kb on + strand, within ECOLIN_RS23175at 4664.665 kb on - strand, within ECOLIN_RS23175at 4664.793 kb on - strand, within ECOLIN_RS23175at 4664.811 kb on - strand, within ECOLIN_RS23175at 4664.811 kb on - strand, within ECOLIN_RS23175at 4664.811 kb on - strand, within ECOLIN_RS23175at 4665.216 kb on + strand, within ECOLIN_RS23175at 4665.310 kb on + strand, within ECOLIN_RS23175at 4665.310 kb on + strand, within ECOLIN_RS23175

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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4,663,060 - ECOLIN_RS23160 0.90 +0.8
4,663,079 + -0.2
4,663,087 - +0.4
4,663,297 - +0.5
4,663,320 + +0.1
4,663,363 - +0.0
4,663,681 + ECOLIN_RS23165 0.57 -1.2
4,664,248 + ECOLIN_RS23170 0.86 -0.1
4,664,375 - +0.9
4,664,539 - -0.1
4,664,664 + ECOLIN_RS23175 0.11 +0.0
4,664,665 - ECOLIN_RS23175 0.11 -0.6
4,664,793 - ECOLIN_RS23175 0.19 +0.1
4,664,811 - ECOLIN_RS23175 0.20 -0.3
4,664,811 - ECOLIN_RS23175 0.20 -0.8
4,664,811 - ECOLIN_RS23175 0.20 -0.1
4,665,216 + ECOLIN_RS23175 0.45 -0.1
4,665,310 + ECOLIN_RS23175 0.51 +0.1
4,665,310 + ECOLIN_RS23175 0.51 -0.2

Or see this region's nucleotide sequence