Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS21630

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS21610 and ECOLIN_RS21615 are separated by 95 nucleotidesECOLIN_RS21615 and ECOLIN_RS21620 are separated by 53 nucleotidesECOLIN_RS21620 and ECOLIN_RS21625 are separated by 8 nucleotidesECOLIN_RS21625 and ECOLIN_RS21630 are separated by 95 nucleotidesECOLIN_RS21630 and ECOLIN_RS21635 are separated by 118 nucleotidesECOLIN_RS21635 and ECOLIN_RS21640 are separated by 24 nucleotides ECOLIN_RS21610: ECOLIN_RS21610 - 23S ribosomal RNA, at 4,341,685 to 4,344,274 _RS21610 ECOLIN_RS21615: ECOLIN_RS21615 - 5S ribosomal RNA, at 4,344,370 to 4,344,485 _RS21615 ECOLIN_RS21620: ECOLIN_RS21620 - tRNA-Asp, at 4,344,539 to 4,344,615 _RS21620 ECOLIN_RS21625: ECOLIN_RS21625 - tRNA-Trp, at 4,344,624 to 4,344,699 _RS21625 ECOLIN_RS21630: ECOLIN_RS21630 - HTH-type transcriptional regulator HdfR, at 4,344,795 to 4,345,634 _RS21630 ECOLIN_RS21635: ECOLIN_RS21635 - macrodomain Ori organization protein MaoP, at 4,345,753 to 4,346,091 _RS21635 ECOLIN_RS21640: ECOLIN_RS21640 - YifB family Mg chelatase-like AAA ATPase, at 4,346,116 to 4,347,637 _RS21640 Position (kb) 4344 4345 4346Strain fitness (log2 ratio) -2 -1 0 1at 4344.829 kb on - strandat 4344.903 kb on - strand, within ECOLIN_RS21630at 4345.091 kb on - strand, within ECOLIN_RS21630at 4345.094 kb on + strand, within ECOLIN_RS21630at 4345.128 kb on - strand, within ECOLIN_RS21630at 4345.196 kb on + strand, within ECOLIN_RS21630at 4345.196 kb on + strand, within ECOLIN_RS21630at 4345.196 kb on + strand, within ECOLIN_RS21630at 4345.200 kb on - strand, within ECOLIN_RS21630at 4345.200 kb on - strand, within ECOLIN_RS21630at 4345.323 kb on - strand, within ECOLIN_RS21630at 4345.328 kb on + strand, within ECOLIN_RS21630at 4345.329 kb on - strand, within ECOLIN_RS21630at 4345.329 kb on - strand, within ECOLIN_RS21630at 4345.370 kb on + strand, within ECOLIN_RS21630at 4345.370 kb on + strand, within ECOLIN_RS21630at 4345.436 kb on - strand, within ECOLIN_RS21630at 4345.669 kb on + strandat 4345.703 kb on + strandat 4345.704 kb on - strandat 4345.704 kb on - strandat 4345.706 kb on + strandat 4345.718 kb on + strandat 4345.718 kb on + strandat 4345.719 kb on - strandat 4345.722 kb on + strandat 4345.739 kb on + strandat 4345.767 kb on + strandat 4345.934 kb on + strand, within ECOLIN_RS21635at 4345.994 kb on - strand, within ECOLIN_RS21635at 4345.995 kb on + strand, within ECOLIN_RS21635at 4346.071 kb on + strandat 4346.071 kb on + strandat 4346.092 kb on + strandat 4346.165 kb on + strandat 4346.170 kb on - strandat 4346.170 kb on - strandat 4346.170 kb on - strandat 4346.170 kb on - strandat 4346.338 kb on + strand, within ECOLIN_RS21640at 4346.339 kb on - strand, within ECOLIN_RS21640at 4346.538 kb on - strand, within ECOLIN_RS21640at 4346.542 kb on + strand, within ECOLIN_RS21640at 4346.543 kb on - strand, within ECOLIN_RS21640

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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4,344,829 - -0.6
4,344,903 - ECOLIN_RS21630 0.13 -0.7
4,345,091 - ECOLIN_RS21630 0.35 -1.2
4,345,094 + ECOLIN_RS21630 0.36 -1.8
4,345,128 - ECOLIN_RS21630 0.40 +1.0
4,345,196 + ECOLIN_RS21630 0.48 -0.5
4,345,196 + ECOLIN_RS21630 0.48 -0.1
4,345,196 + ECOLIN_RS21630 0.48 +0.0
4,345,200 - ECOLIN_RS21630 0.48 -0.8
4,345,200 - ECOLIN_RS21630 0.48 -1.1
4,345,323 - ECOLIN_RS21630 0.63 -0.8
4,345,328 + ECOLIN_RS21630 0.63 -0.7
4,345,329 - ECOLIN_RS21630 0.64 -0.7
4,345,329 - ECOLIN_RS21630 0.64 -1.0
4,345,370 + ECOLIN_RS21630 0.68 -1.3
4,345,370 + ECOLIN_RS21630 0.68 -0.4
4,345,436 - ECOLIN_RS21630 0.76 -1.4
4,345,669 + -0.7
4,345,703 + -0.2
4,345,704 - +0.6
4,345,704 - +0.1
4,345,706 + -2.3
4,345,718 + -0.6
4,345,718 + -0.5
4,345,719 - -0.8
4,345,722 + -0.8
4,345,739 + -0.3
4,345,767 + -1.3
4,345,934 + ECOLIN_RS21635 0.53 -0.6
4,345,994 - ECOLIN_RS21635 0.71 -0.3
4,345,995 + ECOLIN_RS21635 0.71 -1.1
4,346,071 + +0.5
4,346,071 + -0.4
4,346,092 + -0.7
4,346,165 + -0.2
4,346,170 - +0.1
4,346,170 - -0.2
4,346,170 - -0.4
4,346,170 - +0.2
4,346,338 + ECOLIN_RS21640 0.15 +0.1
4,346,339 - ECOLIN_RS21640 0.15 +0.0
4,346,538 - ECOLIN_RS21640 0.28 +0.2
4,346,542 + ECOLIN_RS21640 0.28 +0.2
4,346,543 - ECOLIN_RS21640 0.28 -0.9

Or see this region's nucleotide sequence