Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14055

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14045 and ECOLIN_RS14050 are separated by 12 nucleotidesECOLIN_RS14050 and ECOLIN_RS14055 are separated by 292 nucleotidesECOLIN_RS14055 and ECOLIN_RS14060 are separated by 288 nucleotides ECOLIN_RS14045: ECOLIN_RS14045 - ethanolamine utilization acetate kinase EutP, at 2,817,801 to 2,818,280 _RS14045 ECOLIN_RS14050: ECOLIN_RS14050 - ethanolamine utilization microcompartment protein EutS, at 2,818,293 to 2,818,628 _RS14050 ECOLIN_RS14055: ECOLIN_RS14055 - NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase, at 2,818,921 to 2,821,200 _RS14055 ECOLIN_RS14060: ECOLIN_RS14060 - transaldolase, at 2,821,489 to 2,822,439 _RS14060 Position (kb) 2818 2819 2820 2821 2822Strain fitness (log2 ratio) -1 0 1 2at 2818.073 kb on - strand, within ECOLIN_RS14045at 2820.069 kb on - strand, within ECOLIN_RS14055at 2820.102 kb on - strand, within ECOLIN_RS14055at 2820.558 kb on + strand, within ECOLIN_RS14055at 2820.559 kb on - strand, within ECOLIN_RS14055at 2820.884 kb on - strand, within ECOLIN_RS14055at 2821.220 kb on - strandat 2821.277 kb on - strandat 2821.386 kb on - strandat 2821.401 kb on + strandat 2821.638 kb on - strand, within ECOLIN_RS14060

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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2,818,073 - ECOLIN_RS14045 0.57 -0.2
2,820,069 - ECOLIN_RS14055 0.50 +1.6
2,820,102 - ECOLIN_RS14055 0.52 +2.1
2,820,558 + ECOLIN_RS14055 0.72 +0.2
2,820,559 - ECOLIN_RS14055 0.72 +0.3
2,820,884 - ECOLIN_RS14055 0.86 -0.7
2,821,220 - +0.1
2,821,277 - -0.2
2,821,386 - +0.5
2,821,401 + -0.9
2,821,638 - ECOLIN_RS14060 0.16 +0.7

Or see this region's nucleotide sequence