Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03025

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03020 and ECOLIN_RS03025 are separated by 132 nucleotidesECOLIN_RS03025 and ECOLIN_RS03030 overlap by 4 nucleotidesECOLIN_RS03030 and ECOLIN_RS03035 are separated by 95 nucleotides ECOLIN_RS03020: ECOLIN_RS03020 - carbamate kinase, at 624,356 to 625,249 _RS03020 ECOLIN_RS03025: ECOLIN_RS03025 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 625,382 to 626,449 _RS03025 ECOLIN_RS03030: ECOLIN_RS03030 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 626,446 to 626,955 _RS03030 ECOLIN_RS03035: ECOLIN_RS03035 - hypothetical protein, at 627,051 to 627,380 _RS03035 Position (kb) 625 626 627Strain fitness (log2 ratio) -1 0 1at 624.558 kb on + strand, within ECOLIN_RS03020at 624.579 kb on + strand, within ECOLIN_RS03020at 624.611 kb on + strand, within ECOLIN_RS03020at 624.773 kb on + strand, within ECOLIN_RS03020at 624.773 kb on + strand, within ECOLIN_RS03020at 625.037 kb on + strand, within ECOLIN_RS03020at 625.795 kb on - strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025at 627.315 kb on - strand, within ECOLIN_RS03035at 627.383 kb on + strandat 627.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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624,558 + ECOLIN_RS03020 0.23 +0.4
624,579 + ECOLIN_RS03020 0.25 +0.0
624,611 + ECOLIN_RS03020 0.29 -0.0
624,773 + ECOLIN_RS03020 0.47 -0.2
624,773 + ECOLIN_RS03020 0.47 -0.7
625,037 + ECOLIN_RS03020 0.76 +1.1
625,795 - ECOLIN_RS03025 0.39 +0.8
625,982 + ECOLIN_RS03025 0.56 +0.6
625,982 + ECOLIN_RS03025 0.56 -0.2
627,315 - ECOLIN_RS03035 0.80 -0.3
627,383 + -0.4
627,443 + +0.0

Or see this region's nucleotide sequence