Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02090

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02080 and ECOLIN_RS02085 are separated by 91 nucleotidesECOLIN_RS02085 and ECOLIN_RS02090 are separated by 257 nucleotidesECOLIN_RS02090 and ECOLIN_RS02095 are separated by 39 nucleotidesECOLIN_RS02095 and ECOLIN_RS02100 overlap by 11 nucleotides ECOLIN_RS02080: ECOLIN_RS02080 - LysR substrate-binding domain-containing protein, at 422,469 to 423,401 _RS02080 ECOLIN_RS02085: ECOLIN_RS02085 - DUF1097 domain-containing protein, at 423,493 to 423,990 _RS02085 ECOLIN_RS02090: ECOLIN_RS02090 - ankyrin repeat domain-containing protein, at 424,248 to 424,853 _RS02090 ECOLIN_RS02095: ECOLIN_RS02095 - DUF2877 domain-containing protein, at 424,893 to 425,756 _RS02095 ECOLIN_RS02100: ECOLIN_RS02100 - acyl-CoA synthetase FdrA, at 425,746 to 427,293 _RS02100 Position (kb) 424 425Strain fitness (log2 ratio) -1 0 1at 423.892 kb on + strand, within ECOLIN_RS02085at 424.044 kb on - strandat 424.600 kb on + strand, within ECOLIN_RS02090at 424.733 kb on - strand, within ECOLIN_RS02090at 424.852 kb on - strandat 425.779 kb on + strandat 425.805 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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423,892 + ECOLIN_RS02085 0.80 +0.7
424,044 - -0.9
424,600 + ECOLIN_RS02090 0.58 +0.2
424,733 - ECOLIN_RS02090 0.80 -0.0
424,852 - +0.2
425,779 + +0.6
425,805 + +0.4

Or see this region's nucleotide sequence