Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS27700

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS32255 and ECOLIN_RS01880 are separated by 262 nucleotidesECOLIN_RS01880 and ECOLIN_RS27700 are separated by 70 nucleotidesECOLIN_RS27700 and ECOLIN_RS01885 are separated by 11 nucleotidesECOLIN_RS01885 and ECOLIN_RS01890 are separated by 306 nucleotides ECOLIN_RS32255: ECOLIN_RS32255 - hypothetical protein, at 376,190 to 376,327 _RS32255 ECOLIN_RS01880: ECOLIN_RS01880 - YagU family protein, at 376,590 to 377,204 _RS01880 ECOLIN_RS27700: ECOLIN_RS27700 - tail fiber assembly protein, at 377,275 to 377,441 _RS27700 ECOLIN_RS01885: ECOLIN_RS01885 - YkgJ family cysteine cluster protein, at 377,453 to 377,782 _RS01885 ECOLIN_RS01890: ECOLIN_RS01890 - fimbrial chaperone EcpE, at 378,089 to 378,799 _RS01890 Position (kb) 377 378Strain fitness (log2 ratio) -2 -1 0 1at 376.562 kb on - strandat 376.597 kb on + strandat 376.886 kb on + strand, within ECOLIN_RS01880at 376.957 kb on + strand, within ECOLIN_RS01880at 377.198 kb on + strandat 377.232 kb on + strandat 377.254 kb on - strandat 377.306 kb on + strand, within ECOLIN_RS27700at 377.563 kb on - strand, within ECOLIN_RS01885at 377.782 kb on + strandat 377.793 kb on + strandat 378.090 kb on + strandat 378.159 kb on + strandat 378.212 kb on + strand, within ECOLIN_RS01890

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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376,562 - +0.9
376,597 + +0.1
376,886 + ECOLIN_RS01880 0.48 -0.2
376,957 + ECOLIN_RS01880 0.60 +0.0
377,198 + +0.4
377,232 + -0.2
377,254 - -0.2
377,306 + ECOLIN_RS27700 0.19 +0.7
377,563 - ECOLIN_RS01885 0.33 +0.1
377,782 + -0.2
377,793 + +0.0
378,090 + +0.0
378,159 + -2.1
378,212 + ECOLIN_RS01890 0.17 +0.0

Or see this region's nucleotide sequence