Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS15085

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS15075 and ECOLIN_RS15080 overlap by 4 nucleotidesECOLIN_RS15080 and ECOLIN_RS15085 are separated by 10 nucleotidesECOLIN_RS15085 and ECOLIN_RS15090 are separated by 3 nucleotidesECOLIN_RS15090 and ECOLIN_RS15095 are separated by 105 nucleotides ECOLIN_RS15075: ECOLIN_RS15075 - PTS glucitol/sorbitol transporter subunit IIC, at 3,035,975 to 3,036,538 _RS15075 ECOLIN_RS15080: ECOLIN_RS15080 - PTS glucitol/sorbitol transporter subunit IIB, at 3,036,535 to 3,037,494 _RS15080 ECOLIN_RS15085: ECOLIN_RS15085 - PTS glucitol/sorbitol transporter subunit IIA, at 3,037,505 to 3,037,876 _RS15085 ECOLIN_RS15090: ECOLIN_RS15090 - sorbitol-6-phosphate dehydrogenase, at 3,037,880 to 3,038,659 _RS15090 ECOLIN_RS15095: ECOLIN_RS15095 - transcriptional regulator GutM, at 3,038,765 to 3,039,124 _RS15095 Position (kb) 3037 3038Strain fitness (log2 ratio) -1 0 1 2at 3036.621 kb on + strandat 3036.696 kb on + strand, within ECOLIN_RS15080at 3036.759 kb on - strand, within ECOLIN_RS15080at 3036.856 kb on + strand, within ECOLIN_RS15080at 3036.857 kb on - strand, within ECOLIN_RS15080at 3036.934 kb on + strand, within ECOLIN_RS15080at 3037.483 kb on - strandat 3037.514 kb on - strandat 3037.620 kb on - strand, within ECOLIN_RS15085at 3038.522 kb on + strand, within ECOLIN_RS15090at 3038.522 kb on + strand, within ECOLIN_RS15090at 3038.522 kb on + strand, within ECOLIN_RS15090at 3038.523 kb on - strand, within ECOLIN_RS15090at 3038.523 kb on - strand, within ECOLIN_RS15090at 3038.575 kb on + strand, within ECOLIN_RS15090at 3038.609 kb on + strandat 3038.700 kb on - strandat 3038.716 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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3,036,621 + -1.2
3,036,696 + ECOLIN_RS15080 0.17 -0.3
3,036,759 - ECOLIN_RS15080 0.23 +0.3
3,036,856 + ECOLIN_RS15080 0.33 +1.2
3,036,857 - ECOLIN_RS15080 0.34 +0.8
3,036,934 + ECOLIN_RS15080 0.42 -0.6
3,037,483 - +1.5
3,037,514 - +0.1
3,037,620 - ECOLIN_RS15085 0.31 -0.9
3,038,522 + ECOLIN_RS15090 0.82 -0.9
3,038,522 + ECOLIN_RS15090 0.82 +0.2
3,038,522 + ECOLIN_RS15090 0.82 -0.2
3,038,523 - ECOLIN_RS15090 0.82 +1.2
3,038,523 - ECOLIN_RS15090 0.82 +1.8
3,038,575 + ECOLIN_RS15090 0.89 -0.1
3,038,609 + -0.1
3,038,700 - -0.0
3,038,716 + +0.1

Or see this region's nucleotide sequence