Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12860

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12850 and ECOLIN_RS12855 are separated by 41 nucleotidesECOLIN_RS12855 and ECOLIN_RS12860 overlap by 4 nucleotidesECOLIN_RS12860 and ECOLIN_RS12865 are separated by 12 nucleotidesECOLIN_RS12865 and ECOLIN_RS12870 are separated by 9 nucleotides ECOLIN_RS12850: ECOLIN_RS12850 - heme exporter protein CcmC, at 2,555,146 to 2,555,883 _RS12850 ECOLIN_RS12855: ECOLIN_RS12855 - heme exporter protein CcmB, at 2,555,925 to 2,556,587 _RS12855 ECOLIN_RS12860: ECOLIN_RS12860 - cytochrome c biogenesis heme-transporting ATPase CcmA, at 2,556,584 to 2,557,207 _RS12860 ECOLIN_RS12865: ECOLIN_RS12865 - cytochrome c-type protein NapC, at 2,557,220 to 2,557,822 _RS12865 ECOLIN_RS12870: ECOLIN_RS12870 - nitrate reductase cytochrome c-type subunit, at 2,557,832 to 2,558,281 _RS12870 Position (kb) 2556 2557 2558Strain fitness (log2 ratio) -1 0 1at 2555.971 kb on - strandat 2556.126 kb on - strand, within ECOLIN_RS12855at 2557.357 kb on + strand, within ECOLIN_RS12865at 2557.357 kb on + strand, within ECOLIN_RS12865at 2557.360 kb on - strand, within ECOLIN_RS12865at 2557.502 kb on - strand, within ECOLIN_RS12865at 2557.594 kb on - strand, within ECOLIN_RS12865at 2557.611 kb on + strand, within ECOLIN_RS12865at 2557.649 kb on - strand, within ECOLIN_RS12865

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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2,555,971 - +0.6
2,556,126 - ECOLIN_RS12855 0.30 +0.0
2,557,357 + ECOLIN_RS12865 0.23 +0.9
2,557,357 + ECOLIN_RS12865 0.23 +1.2
2,557,360 - ECOLIN_RS12865 0.23 +0.3
2,557,502 - ECOLIN_RS12865 0.47 +0.5
2,557,594 - ECOLIN_RS12865 0.62 +0.5
2,557,611 + ECOLIN_RS12865 0.65 -0.4
2,557,649 - ECOLIN_RS12865 0.71 +0.2

Or see this region's nucleotide sequence