Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS09685

Experiment: RB51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS09675 and ECOLIN_RS09680 are separated by 7 nucleotidesECOLIN_RS09680 and ECOLIN_RS09685 are separated by 50 nucleotidesECOLIN_RS09685 and ECOLIN_RS09690 are separated by 84 nucleotidesECOLIN_RS09690 and ECOLIN_RS09695 are separated by 299 nucleotides ECOLIN_RS09675: ECOLIN_RS09675 - transcriptional regulator ChbR, at 1,904,761 to 1,905,603 _RS09675 ECOLIN_RS09680: ECOLIN_RS09680 - PTS N,N'-diacetylchitobiose transporter subunit IIA, at 1,905,611 to 1,905,961 _RS09680 ECOLIN_RS09685: ECOLIN_RS09685 - PTS N,N'-diacetylchitobiose transporter subunit IIC, at 1,906,012 to 1,907,370 _RS09685 ECOLIN_RS09690: ECOLIN_RS09690 - PTS N,N'-diacetylchitobiose transporter subunit IIB, at 1,907,455 to 1,907,775 _RS09690 ECOLIN_RS09695: ECOLIN_RS09695 - osmotically-inducible lipoprotein OsmE, at 1,908,075 to 1,908,413 _RS09695 Position (kb) 1906 1907 1908Strain fitness (log2 ratio) -1 0 1at 1905.977 kb on - strandat 1906.847 kb on - strand, within ECOLIN_RS09685at 1906.980 kb on - strand, within ECOLIN_RS09685at 1907.589 kb on + strand, within ECOLIN_RS09690

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Per-strain Table

Position Strand Gene LocusTag Fraction RB51
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1,905,977 - +0.5
1,906,847 - ECOLIN_RS09685 0.61 +0.5
1,906,980 - ECOLIN_RS09685 0.71 -0.6
1,907,589 + ECOLIN_RS09690 0.42 +0.7

Or see this region's nucleotide sequence