Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02375

Experiment: Bas51

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02370 and ECOLIN_RS02375 are separated by 71 nucleotidesECOLIN_RS02375 and ECOLIN_RS02380 are separated by 147 nucleotidesECOLIN_RS02380 and ECOLIN_RS31260 overlap by 4 nucleotidesECOLIN_RS31260 and ECOLIN_RS02385 are separated by 80 nucleotides ECOLIN_RS02370: ECOLIN_RS02370 - AroM family protein, at 482,221 to 482,898 _RS02370 ECOLIN_RS02375: ECOLIN_RS02375 - pyrimidine/purine nucleoside phosphorylase, at 482,970 to 483,254 _RS02375 ECOLIN_RS02380: ECOLIN_RS02380 - DUF2773 domain-containing protein, at 483,402 to 483,743 _RS02380 ECOLIN_RS31260: ECOLIN_RS31260 - hypothetical protein, at 483,740 to 483,820 _RS31260 ECOLIN_RS02385: ECOLIN_RS02385 - recombination-associated protein RdgC, at 483,901 to 484,812 _RS02385 Position (kb) 482 483 484Strain fitness (log2 ratio) -1 0 1at 482.151 kb on + strandat 482.151 kb on + strandat 482.405 kb on + strand, within ECOLIN_RS02370at 482.529 kb on + strand, within ECOLIN_RS02370at 482.753 kb on + strand, within ECOLIN_RS02370at 483.072 kb on + strand, within ECOLIN_RS02375at 483.133 kb on + strand, within ECOLIN_RS02375at 483.576 kb on - strand, within ECOLIN_RS02380

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas51
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482,151 + -0.2
482,151 + +0.2
482,405 + ECOLIN_RS02370 0.27 -1.1
482,529 + ECOLIN_RS02370 0.45 +0.9
482,753 + ECOLIN_RS02370 0.78 +1.0
483,072 + ECOLIN_RS02375 0.36 -0.5
483,133 + ECOLIN_RS02375 0.57 -0.1
483,576 - ECOLIN_RS02380 0.51 -0.3

Or see this region's nucleotide sequence