Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS28115

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS05820 and ECOLIN_RS05825 are separated by 106 nucleotidesECOLIN_RS05825 and ECOLIN_RS28115 are separated by 60 nucleotidesECOLIN_RS28115 and ECOLIN_RS33385 are separated by 1 nucleotidesECOLIN_RS33385 and ECOLIN_RS05830 are separated by 94 nucleotides ECOLIN_RS05820: ECOLIN_RS05820 - GTP-binding protein, at 1,189,763 to 1,190,962 _RS05820 ECOLIN_RS05825: ECOLIN_RS05825 - transposase domain-containing protein, at 1,191,069 to 1,191,254 _RS05825 ECOLIN_RS28115: ECOLIN_RS28115 - IS110 family transposase, at 1,191,315 to 1,191,636 _RS28115 ECOLIN_RS33385: ECOLIN_RS33385 - DUF6404 family protein, at 1,191,638 to 1,191,818 _RS33385 ECOLIN_RS05830: ECOLIN_RS05830 - SEL1-like repeat protein, at 1,191,913 to 1,193,049 _RS05830 Position (kb) 1191 1192Strain fitness (log2 ratio) -1 0 1at 1191.694 kb on - strand, within ECOLIN_RS33385at 1191.829 kb on + strandat 1192.066 kb on + strand, within ECOLIN_RS05830

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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1,191,694 - ECOLIN_RS33385 0.31 +0.5
1,191,829 + +1.3
1,192,066 + ECOLIN_RS05830 0.13 +1.2

Or see this region's nucleotide sequence