Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17580

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17570 and ECOLIN_RS17575 are separated by 15 nucleotidesECOLIN_RS17575 and ECOLIN_RS17580 overlap by 4 nucleotidesECOLIN_RS17580 and ECOLIN_RS17585 are separated by 53 nucleotides ECOLIN_RS17570: ECOLIN_RS17570 - fimbria/pilus outer membrane usher protein, at 3,538,974 to 3,541,496 _RS17570 ECOLIN_RS17575: ECOLIN_RS17575 - fimbria/pilus periplasmic chaperone, at 3,541,512 to 3,542,252 _RS17575 ECOLIN_RS17580: ECOLIN_RS17580 - pilus protein, at 3,542,249 to 3,543,298 _RS17580 ECOLIN_RS17585: ECOLIN_RS17585 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 3,543,352 to 3,544,005 _RS17585 Position (kb) 3542 3543 3544Strain fitness (log2 ratio) -1 0 1at 3541.298 kb on - strandat 3541.298 kb on - strandat 3541.366 kb on - strandat 3541.366 kb on - strandat 3541.402 kb on + strandat 3541.748 kb on + strand, within ECOLIN_RS17575at 3541.803 kb on - strand, within ECOLIN_RS17575at 3541.833 kb on + strand, within ECOLIN_RS17575at 3541.836 kb on + strand, within ECOLIN_RS17575at 3541.837 kb on - strand, within ECOLIN_RS17575at 3542.199 kb on - strandat 3542.225 kb on + strandat 3542.269 kb on - strandat 3542.428 kb on + strand, within ECOLIN_RS17580at 3543.063 kb on + strand, within ECOLIN_RS17580at 3543.064 kb on - strand, within ECOLIN_RS17580at 3543.065 kb on + strand, within ECOLIN_RS17580at 3543.222 kb on + strandat 3543.276 kb on + strandat 3543.280 kb on + strandat 3543.341 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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3,541,298 - +0.6
3,541,298 - +0.9
3,541,366 - -0.1
3,541,366 - +0.6
3,541,402 + -0.1
3,541,748 + ECOLIN_RS17575 0.32 +0.0
3,541,803 - ECOLIN_RS17575 0.39 -1.0
3,541,833 + ECOLIN_RS17575 0.43 -0.6
3,541,836 + ECOLIN_RS17575 0.44 +0.4
3,541,837 - ECOLIN_RS17575 0.44 -0.1
3,542,199 - -0.6
3,542,225 + +0.3
3,542,269 - +0.5
3,542,428 + ECOLIN_RS17580 0.17 +1.5
3,543,063 + ECOLIN_RS17580 0.78 +1.2
3,543,064 - ECOLIN_RS17580 0.78 -0.0
3,543,065 + ECOLIN_RS17580 0.78 +1.2
3,543,222 + -0.3
3,543,276 + +0.6
3,543,280 + +0.1
3,543,341 - -0.3

Or see this region's nucleotide sequence