Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS08965

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS08960 and ECOLIN_RS08965 are separated by 185 nucleotidesECOLIN_RS08965 and ECOLIN_RS08970 are separated by 36 nucleotidesECOLIN_RS08970 and ECOLIN_RS08975 overlap by 4 nucleotides ECOLIN_RS08960: ECOLIN_RS08960 - DUF1471 family protein YdgH, at 1,767,179 to 1,768,123 _RS08960 ECOLIN_RS08965: ECOLIN_RS08965 - amino acid permease, at 1,768,309 to 1,769,691 _RS08965 ECOLIN_RS08970: ECOLIN_RS08970 - dihydromonapterin reductase, at 1,769,728 to 1,770,450 _RS08970 ECOLIN_RS08975: ECOLIN_RS08975 - GlpM family protein, at 1,770,447 to 1,770,782 _RS08975 Position (kb) 1768 1769 1770Strain fitness (log2 ratio) -1 0 1at 1768.118 kb on - strandat 1768.118 kb on - strandat 1768.139 kb on + strandat 1769.501 kb on + strand, within ECOLIN_RS08965at 1770.458 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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1,768,118 - +0.0
1,768,118 - +0.6
1,768,139 + +0.5
1,769,501 + ECOLIN_RS08965 0.86 -1.7
1,770,458 + +0.5

Or see this region's nucleotide sequence