Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20280

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20270 and ECOLIN_RS32110 are separated by 17 nucleotidesECOLIN_RS32110 and ECOLIN_RS20280 are separated by 61 nucleotidesECOLIN_RS20280 and ECOLIN_RS20290 are separated by 743 nucleotidesECOLIN_RS20290 and ECOLIN_RS20295 are separated by 91 nucleotides ECOLIN_RS20270: ECOLIN_RS20270 - dipeptide ABC transporter permease DppB, at 4,077,309 to 4,078,328 _RS20270 ECOLIN_RS32110: ECOLIN_RS32110 - hypothetical protein, at 4,078,346 to 4,078,573 _RS32110 ECOLIN_RS20280: ECOLIN_RS20280 - dipeptide ABC transporter substrate-binding protein DppA, at 4,078,635 to 4,080,242 _RS20280 ECOLIN_RS20290: ECOLIN_RS20290 - tRNA-Pro, at 4,080,986 to 4,081,062 _RS20290 ECOLIN_RS20295: ECOLIN_RS20295 - kdo(2)-lipid A phosphoethanolamine 7''-transferase, at 4,081,154 to 4,082,845 _RS20295 Position (kb) 4078 4079 4080 4081Strain fitness (log2 ratio) -1 0 1at 4078.111 kb on - strand, within ECOLIN_RS20270at 4078.132 kb on + strand, within ECOLIN_RS20270at 4078.133 kb on - strand, within ECOLIN_RS20270at 4078.155 kb on + strand, within ECOLIN_RS20270at 4078.272 kb on - strandat 4078.342 kb on + strandat 4078.343 kb on - strandat 4078.624 kb on + strandat 4078.625 kb on - strandat 4078.632 kb on + strandat 4078.687 kb on + strandat 4078.852 kb on + strand, within ECOLIN_RS20280at 4078.852 kb on + strand, within ECOLIN_RS20280at 4078.853 kb on - strand, within ECOLIN_RS20280at 4079.008 kb on + strand, within ECOLIN_RS20280at 4079.009 kb on - strand, within ECOLIN_RS20280at 4079.182 kb on + strand, within ECOLIN_RS20280at 4079.182 kb on + strand, within ECOLIN_RS20280at 4079.209 kb on + strand, within ECOLIN_RS20280at 4079.351 kb on + strand, within ECOLIN_RS20280at 4079.426 kb on + strand, within ECOLIN_RS20280at 4079.504 kb on + strand, within ECOLIN_RS20280at 4079.504 kb on + strand, within ECOLIN_RS20280at 4079.507 kb on + strand, within ECOLIN_RS20280at 4079.508 kb on - strand, within ECOLIN_RS20280at 4079.564 kb on + strand, within ECOLIN_RS20280at 4079.566 kb on + strand, within ECOLIN_RS20280at 4079.691 kb on - strand, within ECOLIN_RS20280at 4079.790 kb on + strand, within ECOLIN_RS20280at 4079.790 kb on + strand, within ECOLIN_RS20280at 4079.791 kb on - strand, within ECOLIN_RS20280at 4079.889 kb on + strand, within ECOLIN_RS20280at 4079.890 kb on - strand, within ECOLIN_RS20280at 4079.970 kb on - strand, within ECOLIN_RS20280at 4079.970 kb on - strand, within ECOLIN_RS20280at 4079.971 kb on + strand, within ECOLIN_RS20280at 4079.972 kb on - strand, within ECOLIN_RS20280at 4080.062 kb on - strand, within ECOLIN_RS20280at 4080.085 kb on + strandat 4080.085 kb on + strandat 4080.117 kb on - strandat 4080.339 kb on - strandat 4080.571 kb on + strandat 4080.588 kb on + strandat 4080.632 kb on + strandat 4080.644 kb on + strandat 4080.694 kb on - strandat 4080.810 kb on + strandat 4080.943 kb on - strandat 4081.155 kb on + strandat 4081.155 kb on + strandat 4081.231 kb on + strandat 4081.231 kb on + strandat 4081.232 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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4,078,111 - ECOLIN_RS20270 0.79 -0.1
4,078,132 + ECOLIN_RS20270 0.81 -0.1
4,078,133 - ECOLIN_RS20270 0.81 +0.3
4,078,155 + ECOLIN_RS20270 0.83 -0.1
4,078,272 - -0.4
4,078,342 + +0.1
4,078,343 - -0.0
4,078,624 + +0.3
4,078,625 - -0.3
4,078,632 + +0.9
4,078,687 + -0.1
4,078,852 + ECOLIN_RS20280 0.13 -0.1
4,078,852 + ECOLIN_RS20280 0.13 +0.2
4,078,853 - ECOLIN_RS20280 0.14 +0.0
4,079,008 + ECOLIN_RS20280 0.23 -0.1
4,079,009 - ECOLIN_RS20280 0.23 -0.2
4,079,182 + ECOLIN_RS20280 0.34 +0.2
4,079,182 + ECOLIN_RS20280 0.34 +0.1
4,079,209 + ECOLIN_RS20280 0.36 -0.7
4,079,351 + ECOLIN_RS20280 0.45 +0.3
4,079,426 + ECOLIN_RS20280 0.49 -0.1
4,079,504 + ECOLIN_RS20280 0.54 +0.2
4,079,504 + ECOLIN_RS20280 0.54 -0.7
4,079,507 + ECOLIN_RS20280 0.54 -0.1
4,079,508 - ECOLIN_RS20280 0.54 -0.4
4,079,564 + ECOLIN_RS20280 0.58 -0.1
4,079,566 + ECOLIN_RS20280 0.58 +0.2
4,079,691 - ECOLIN_RS20280 0.66 -0.9
4,079,790 + ECOLIN_RS20280 0.72 +0.1
4,079,790 + ECOLIN_RS20280 0.72 +0.0
4,079,791 - ECOLIN_RS20280 0.72 -0.2
4,079,889 + ECOLIN_RS20280 0.78 -0.5
4,079,890 - ECOLIN_RS20280 0.78 +1.7
4,079,970 - ECOLIN_RS20280 0.83 -0.2
4,079,970 - ECOLIN_RS20280 0.83 -0.5
4,079,971 + ECOLIN_RS20280 0.83 -0.0
4,079,972 - ECOLIN_RS20280 0.83 +0.2
4,080,062 - ECOLIN_RS20280 0.89 +0.2
4,080,085 + -0.7
4,080,085 + -1.1
4,080,117 - +0.6
4,080,339 - +0.2
4,080,571 + +0.8
4,080,588 + -0.2
4,080,632 + -0.6
4,080,644 + -0.2
4,080,694 - +0.3
4,080,810 + -0.7
4,080,943 - -0.3
4,081,155 + -0.2
4,081,155 + +0.2
4,081,231 + +0.0
4,081,231 + +0.9
4,081,232 - +0.4

Or see this region's nucleotide sequence