Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13150

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13145 and ECOLIN_RS13150 overlap by 4 nucleotidesECOLIN_RS13150 and ECOLIN_RS13155 overlap by 1 nucleotidesECOLIN_RS13155 and ECOLIN_RS13160 overlap by 7 nucleotidesECOLIN_RS13160 and ECOLIN_RS13165 are separated by 109 nucleotides ECOLIN_RS13145: ECOLIN_RS13145 - lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase, at 2,629,345 to 2,630,997 _RS13145 ECOLIN_RS13150: ECOLIN_RS13150 - 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE, at 2,630,994 to 2,631,329 _RS13150 ECOLIN_RS13155: ECOLIN_RS13155 - 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnF, at 2,631,329 to 2,631,715 _RS13155 ECOLIN_RS13160: ECOLIN_RS13160 - signal transduction protein PmrD, at 2,631,709 to 2,631,975 _RS13160 ECOLIN_RS13165: ECOLIN_RS13165 - o-succinylbenzoate--CoA ligase, at 2,632,085 to 2,633,440 _RS13165 Position (kb) 2630 2631 2632Strain fitness (log2 ratio) -1 0 1at 2630.287 kb on + strand, within ECOLIN_RS13145at 2630.288 kb on - strand, within ECOLIN_RS13145at 2630.288 kb on - strand, within ECOLIN_RS13145at 2630.791 kb on + strand, within ECOLIN_RS13145at 2631.098 kb on + strand, within ECOLIN_RS13150at 2631.348 kb on + strandat 2631.733 kb on - strandat 2631.811 kb on - strand, within ECOLIN_RS13160at 2631.919 kb on - strand, within ECOLIN_RS13160at 2631.923 kb on - strand, within ECOLIN_RS13160

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,630,287 + ECOLIN_RS13145 0.57 -0.5
2,630,288 - ECOLIN_RS13145 0.57 -0.3
2,630,288 - ECOLIN_RS13145 0.57 +1.7
2,630,791 + ECOLIN_RS13145 0.87 -0.8
2,631,098 + ECOLIN_RS13150 0.31 +0.7
2,631,348 + -0.0
2,631,733 - +0.7
2,631,811 - ECOLIN_RS13160 0.38 -0.1
2,631,919 - ECOLIN_RS13160 0.79 -0.2
2,631,923 - ECOLIN_RS13160 0.80 -0.8

Or see this region's nucleotide sequence