Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12150

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12145 and ECOLIN_RS12150 are separated by 67 nucleotidesECOLIN_RS12150 and ECOLIN_RS12155 are separated by 21 nucleotidesECOLIN_RS12155 and ECOLIN_RS12160 are separated by 91 nucleotides ECOLIN_RS12145: ECOLIN_RS12145 - TerC family protein, at 2,398,611 to 2,400,194 _RS12145 ECOLIN_RS12150: ECOLIN_RS12150 - outer membrane assembly protein AsmA, at 2,400,262 to 2,402,115 _RS12150 ECOLIN_RS12155: ECOLIN_RS12155 - dCTP deaminase, at 2,402,137 to 2,402,718 _RS12155 ECOLIN_RS12160: ECOLIN_RS12160 - uridine kinase, at 2,402,810 to 2,403,451 _RS12160 Position (kb) 2400 2401 2402 2403Strain fitness (log2 ratio) -1 0 1at 2401.933 kb on - strandat 2402.016 kb on - strandat 2402.475 kb on - strand, within ECOLIN_RS12155at 2402.903 kb on - strand, within ECOLIN_RS12160

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,401,933 - -0.0
2,402,016 - +1.3
2,402,475 - ECOLIN_RS12155 0.58 -0.1
2,402,903 - ECOLIN_RS12160 0.14 +0.3

Or see this region's nucleotide sequence