Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS02430 and ECOLIN_RS02435 are separated by 4 nucleotides ECOLIN_RS02435 and ECOLIN_RS02440 are separated by 218 nucleotides ECOLIN_RS02440 and ECOLIN_RS02445 are separated by 54 nucleotides
ECOLIN_RS02430: ECOLIN_RS02430 - maltodextrin glucosidase, at 497,329 to 499,146
_RS02430
ECOLIN_RS02435: ECOLIN_RS02435 - ACP phosphodiesterase, at 499,151 to 499,732
_RS02435
ECOLIN_RS02440: ECOLIN_RS02440 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 499,951 to 501,021
_RS02440
ECOLIN_RS02445: ECOLIN_RS02445 - tRNA guanosine(34) transglycosylase Tgt, at 501,076 to 502,203
_RS02445
Position (kb) 499
500
501
502 Strain fitness (log2 ratio) -1
0
1 at 499.152 kb on + strand at 499.430 kb on - strand, within ECOLIN_RS02435 at 499.511 kb on - strand, within ECOLIN_RS02435 at 499.584 kb on - strand, within ECOLIN_RS02435 at 499.603 kb on + strand, within ECOLIN_RS02435 at 499.604 kb on - strand, within ECOLIN_RS02435 at 499.791 kb on + strand at 499.792 kb on - strand at 499.801 kb on - strand at 499.801 kb on - strand at 499.801 kb on - strand at 499.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 499,152 + -0.2 499,430 - ECOLIN_RS02435 0.48 -0.3 499,511 - ECOLIN_RS02435 0.62 +1.3 499,584 - ECOLIN_RS02435 0.74 +1.2 499,603 + ECOLIN_RS02435 0.78 +1.0 499,604 - ECOLIN_RS02435 0.78 -0.2 499,791 + +0.7 499,792 - +0.3 499,801 - -0.7 499,801 - -0.6 499,801 - -0.1 499,864 - -0.3
Or see this region's nucleotide sequence