Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02420

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02415 and ECOLIN_RS02420 are separated by 406 nucleotidesECOLIN_RS02420 and ECOLIN_RS02425 are separated by 75 nucleotides ECOLIN_RS02415: ECOLIN_RS02415 - phosphate regulon sensor histidine kinase PhoR, at 492,703 to 493,998 _RS02415 ECOLIN_RS02420: ECOLIN_RS02420 - branched-chain amino acid transporter carrier protein BrnQ, at 494,405 to 495,724 _RS02420 ECOLIN_RS02425: ECOLIN_RS02425 - proline-specific permease ProY, at 495,800 to 497,173 _RS02425 Position (kb) 494 495 496Strain fitness (log2 ratio) -1 0 1 2 3 4at 493.499 kb on + strand, within ECOLIN_RS02415at 493.499 kb on + strand, within ECOLIN_RS02415at 493.725 kb on - strand, within ECOLIN_RS02415at 494.145 kb on - strandat 494.330 kb on - strandat 494.331 kb on + strandat 494.485 kb on + strandat 494.905 kb on + strand, within ECOLIN_RS02420at 495.021 kb on + strand, within ECOLIN_RS02420at 495.329 kb on + strand, within ECOLIN_RS02420at 495.831 kb on + strandat 495.846 kb on + strandat 495.846 kb on + strandat 495.853 kb on + strandat 495.890 kb on + strandat 495.890 kb on + strandat 495.965 kb on + strand, within ECOLIN_RS02425at 496.082 kb on + strand, within ECOLIN_RS02425at 496.136 kb on + strand, within ECOLIN_RS02425at 496.136 kb on + strand, within ECOLIN_RS02425at 496.136 kb on + strand, within ECOLIN_RS02425at 496.136 kb on + strand, within ECOLIN_RS02425at 496.137 kb on - strand, within ECOLIN_RS02425at 496.561 kb on + strand, within ECOLIN_RS02425at 496.568 kb on - strand, within ECOLIN_RS02425at 496.693 kb on + strand, within ECOLIN_RS02425

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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493,499 + ECOLIN_RS02415 0.61 -0.3
493,499 + ECOLIN_RS02415 0.61 +1.6
493,725 - ECOLIN_RS02415 0.79 +0.6
494,145 - -0.6
494,330 - -0.6
494,331 + +0.1
494,485 + -0.6
494,905 + ECOLIN_RS02420 0.38 -0.1
495,021 + ECOLIN_RS02420 0.47 +0.6
495,329 + ECOLIN_RS02420 0.70 -1.1
495,831 + -0.2
495,846 + -0.5
495,846 + -0.3
495,853 + +0.4
495,890 + +1.2
495,890 + +0.0
495,965 + ECOLIN_RS02425 0.12 +0.0
496,082 + ECOLIN_RS02425 0.21 -0.8
496,136 + ECOLIN_RS02425 0.24 +0.0
496,136 + ECOLIN_RS02425 0.24 +1.2
496,136 + ECOLIN_RS02425 0.24 +0.4
496,136 + ECOLIN_RS02425 0.24 +3.8
496,137 - ECOLIN_RS02425 0.25 +1.6
496,561 + ECOLIN_RS02425 0.55 +0.2
496,568 - ECOLIN_RS02425 0.56 +0.6
496,693 + ECOLIN_RS02425 0.65 +0.2

Or see this region's nucleotide sequence