Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20595

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20585 and ECOLIN_RS20590 are separated by 54 nucleotidesECOLIN_RS20590 and ECOLIN_RS20595 are separated by 79 nucleotidesECOLIN_RS20595 and ECOLIN_RS20600 overlap by 1 nucleotidesECOLIN_RS20600 and ECOLIN_RS20605 are separated by 63 nucleotidesECOLIN_RS20605 and ECOLIN_RS20610 are separated by 140 nucleotides ECOLIN_RS20585: ECOLIN_RS20585 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 4,148,731 to 4,149,204 _RS20585 ECOLIN_RS20590: ECOLIN_RS20590 - serine O-acetyltransferase, at 4,149,259 to 4,150,080 _RS20590 ECOLIN_RS20595: ECOLIN_RS20595 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 4,150,160 to 4,151,179 _RS20595 ECOLIN_RS20600: ECOLIN_RS20600 - protein-export chaperone SecB, at 4,151,179 to 4,151,646 _RS20600 ECOLIN_RS20605: ECOLIN_RS20605 - glutaredoxin 3, at 4,151,710 to 4,151,961 _RS20605 ECOLIN_RS20610: ECOLIN_RS20610 - rhodanese-like domain-containing protein, at 4,152,102 to 4,152,533 _RS20610 Position (kb) 4150 4151 4152Strain fitness (log2 ratio) -2 -1 0 1at 4149.275 kb on + strandat 4149.286 kb on - strandat 4149.315 kb on + strandat 4149.559 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590at 4149.770 kb on + strand, within ECOLIN_RS20590at 4149.772 kb on + strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.792 kb on + strand, within ECOLIN_RS20590at 4149.828 kb on + strand, within ECOLIN_RS20590at 4149.909 kb on + strand, within ECOLIN_RS20590at 4149.910 kb on - strand, within ECOLIN_RS20590at 4149.941 kb on + strand, within ECOLIN_RS20590at 4149.941 kb on + strand, within ECOLIN_RS20590at 4149.942 kb on - strand, within ECOLIN_RS20590at 4150.041 kb on + strandat 4150.044 kb on + strandat 4150.085 kb on + strandat 4150.103 kb on - strandat 4151.675 kb on - strandat 4151.692 kb on - strandat 4151.692 kb on - strandat 4151.692 kb on - strandat 4151.754 kb on - strand, within ECOLIN_RS20605at 4151.966 kb on + strandat 4152.036 kb on - strandat 4152.037 kb on + strandat 4152.037 kb on + strandat 4152.038 kb on - strandat 4152.038 kb on - strandat 4152.044 kb on - strandat 4152.104 kb on - strandat 4152.163 kb on - strand, within ECOLIN_RS20610at 4152.169 kb on - strand, within ECOLIN_RS20610

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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4,149,275 + +0.1
4,149,286 - -0.2
4,149,315 + +0.3
4,149,559 + ECOLIN_RS20590 0.36 -0.8
4,149,649 + ECOLIN_RS20590 0.47 -0.4
4,149,649 + ECOLIN_RS20590 0.47 -1.0
4,149,649 + ECOLIN_RS20590 0.47 -0.5
4,149,649 + ECOLIN_RS20590 0.47 -0.4
4,149,650 - ECOLIN_RS20590 0.48 +1.3
4,149,650 - ECOLIN_RS20590 0.48 -1.1
4,149,770 + ECOLIN_RS20590 0.62 -1.0
4,149,772 + ECOLIN_RS20590 0.62 -0.2
4,149,789 - ECOLIN_RS20590 0.64 -1.4
4,149,789 - ECOLIN_RS20590 0.64 -0.5
4,149,789 - ECOLIN_RS20590 0.64 -0.9
4,149,792 + ECOLIN_RS20590 0.65 -0.2
4,149,828 + ECOLIN_RS20590 0.69 -0.3
4,149,909 + ECOLIN_RS20590 0.79 -0.7
4,149,910 - ECOLIN_RS20590 0.79 -0.4
4,149,941 + ECOLIN_RS20590 0.83 -1.7
4,149,941 + ECOLIN_RS20590 0.83 -2.2
4,149,942 - ECOLIN_RS20590 0.83 +0.6
4,150,041 + -0.5
4,150,044 + +0.5
4,150,085 + -0.0
4,150,103 - -0.3
4,151,675 - +0.0
4,151,692 - +0.1
4,151,692 - +0.8
4,151,692 - -0.7
4,151,754 - ECOLIN_RS20605 0.17 +0.1
4,151,966 + +0.7
4,152,036 - -0.2
4,152,037 + +0.2
4,152,037 + +1.0
4,152,038 - +0.1
4,152,038 - -0.3
4,152,044 - +0.3
4,152,104 - +0.2
4,152,163 - ECOLIN_RS20610 0.14 -0.3
4,152,169 - ECOLIN_RS20610 0.16 -0.0

Or see this region's nucleotide sequence