Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14895

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14885 and ECOLIN_RS14890 are separated by 36 nucleotidesECOLIN_RS14890 and ECOLIN_RS14895 are separated by 151 nucleotidesECOLIN_RS14895 and ECOLIN_RS14900 are separated by 56 nucleotides ECOLIN_RS14885: ECOLIN_RS14885 - L-alanine exporter AlaE, at 3,005,505 to 3,005,954 _RS14885 ECOLIN_RS14890: ECOLIN_RS14890 - DUF2002 family protein, at 3,005,991 to 3,006,335 _RS14890 ECOLIN_RS14895: ECOLIN_RS14895 - DUF883 domain-containing protein, at 3,006,487 to 3,006,816 _RS14895 ECOLIN_RS14900: ECOLIN_RS14900 - PLP-dependent aminotransferase family protein, at 3,006,873 to 3,008,207 _RS14900 Position (kb) 3006 3007Strain fitness (log2 ratio) -1 0 1at 3005.665 kb on + strand, within ECOLIN_RS14885at 3005.766 kb on + strand, within ECOLIN_RS14885at 3005.790 kb on + strand, within ECOLIN_RS14885at 3005.892 kb on + strand, within ECOLIN_RS14885at 3006.233 kb on - strand, within ECOLIN_RS14890at 3006.331 kb on + strandat 3006.352 kb on + strandat 3006.353 kb on - strandat 3006.449 kb on + strandat 3006.880 kb on - strandat 3006.887 kb on + strandat 3007.421 kb on - strand, within ECOLIN_RS14900at 3007.449 kb on - strand, within ECOLIN_RS14900at 3007.714 kb on - strand, within ECOLIN_RS14900

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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3,005,665 + ECOLIN_RS14885 0.36 -0.2
3,005,766 + ECOLIN_RS14885 0.58 +0.1
3,005,790 + ECOLIN_RS14885 0.63 +0.5
3,005,892 + ECOLIN_RS14885 0.86 +0.1
3,006,233 - ECOLIN_RS14890 0.70 -0.1
3,006,331 + -1.4
3,006,352 + -0.8
3,006,353 - -0.2
3,006,449 + -0.9
3,006,880 - +0.1
3,006,887 + +1.2
3,007,421 - ECOLIN_RS14900 0.41 +0.5
3,007,449 - ECOLIN_RS14900 0.43 +0.3
3,007,714 - ECOLIN_RS14900 0.63 +0.7

Or see this region's nucleotide sequence