Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12715

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12710 and ECOLIN_RS12715 are separated by 38 nucleotidesECOLIN_RS12715 and ECOLIN_RS12720 are separated by 411 nucleotides ECOLIN_RS12710: ECOLIN_RS12710 - zinc-binding GTPase YeiR, at 2,530,419 to 2,531,405 _RS12710 ECOLIN_RS12715: ECOLIN_RS12715 - Kdo(2)-lipid A phosphotransferase, at 2,531,444 to 2,532,157 _RS12715 ECOLIN_RS12720: ECOLIN_RS12720 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 2,532,569 to 2,533,135 _RS12720 Position (kb) 2531 2532 2533Strain fitness (log2 ratio) -1 0 1at 2532.294 kb on - strandat 2532.436 kb on + strandat 2532.598 kb on + strandat 2532.649 kb on + strand, within ECOLIN_RS12720at 2532.969 kb on + strand, within ECOLIN_RS12720at 2533.013 kb on + strand, within ECOLIN_RS12720at 2533.013 kb on + strand, within ECOLIN_RS12720at 2533.063 kb on + strand, within ECOLIN_RS12720

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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2,532,294 - +0.4
2,532,436 + +0.1
2,532,598 + +0.1
2,532,649 + ECOLIN_RS12720 0.14 +0.5
2,532,969 + ECOLIN_RS12720 0.71 -0.2
2,533,013 + ECOLIN_RS12720 0.78 +0.1
2,533,013 + ECOLIN_RS12720 0.78 +0.1
2,533,063 + ECOLIN_RS12720 0.87 +0.3

Or see this region's nucleotide sequence