Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS22385

Experiment: Bas45

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS22375 and ECOLIN_RS22380 overlap by 7 nucleotidesECOLIN_RS22380 and ECOLIN_RS22385 overlap by 4 nucleotidesECOLIN_RS22385 and ECOLIN_RS22390 are separated by 44 nucleotides ECOLIN_RS22375: ECOLIN_RS22375 - glucose-1-phosphatase, at 4,498,901 to 4,499,500 _RS22375 ECOLIN_RS22380: ECOLIN_RS22380 - virulence factor BrkB family protein, at 4,499,494 to 4,500,366 _RS22380 ECOLIN_RS22385: ECOLIN_RS22385 - D-aminoacyl-tRNA deacylase, at 4,500,363 to 4,500,800 _RS22385 ECOLIN_RS22390: ECOLIN_RS22390 - fatty acid biosynthesis protein FabY, at 4,500,845 to 4,501,786 _RS22390 Position (kb) 4500 4501Strain fitness (log2 ratio) -1 0 1 2at 4499.411 kb on - strand, within ECOLIN_RS22375at 4499.411 kb on - strand, within ECOLIN_RS22375at 4499.411 kb on - strand, within ECOLIN_RS22375at 4499.413 kb on - strand, within ECOLIN_RS22375at 4499.413 kb on - strand, within ECOLIN_RS22375at 4499.422 kb on + strand, within ECOLIN_RS22375at 4499.440 kb on + strand, within ECOLIN_RS22375at 4499.440 kb on + strand, within ECOLIN_RS22375at 4499.440 kb on + strand, within ECOLIN_RS22375at 4499.443 kb on + strandat 4499.443 kb on + strandat 4499.494 kb on - strandat 4499.498 kb on + strandat 4499.499 kb on - strandat 4499.553 kb on - strandat 4499.615 kb on - strand, within ECOLIN_RS22380at 4499.615 kb on - strand, within ECOLIN_RS22380at 4499.845 kb on + strand, within ECOLIN_RS22380at 4500.040 kb on - strand, within ECOLIN_RS22380at 4500.096 kb on + strand, within ECOLIN_RS22380at 4500.097 kb on - strand, within ECOLIN_RS22380at 4500.097 kb on - strand, within ECOLIN_RS22380at 4500.108 kb on - strand, within ECOLIN_RS22380at 4500.108 kb on - strand, within ECOLIN_RS22380at 4500.205 kb on + strand, within ECOLIN_RS22380at 4500.217 kb on + strand, within ECOLIN_RS22380at 4500.273 kb on + strand, within ECOLIN_RS22380at 4500.297 kb on + strandat 4500.373 kb on + strandat 4500.373 kb on + strandat 4500.580 kb on + strand, within ECOLIN_RS22385at 4500.581 kb on - strand, within ECOLIN_RS22385at 4500.581 kb on - strand, within ECOLIN_RS22385at 4500.676 kb on + strand, within ECOLIN_RS22385at 4500.678 kb on + strand, within ECOLIN_RS22385at 4500.678 kb on + strand, within ECOLIN_RS22385at 4500.678 kb on + strand, within ECOLIN_RS22385at 4500.678 kb on + strand, within ECOLIN_RS22385at 4500.679 kb on - strand, within ECOLIN_RS22385at 4500.679 kb on - strand, within ECOLIN_RS22385at 4500.679 kb on - strand, within ECOLIN_RS22385at 4500.679 kb on - strand, within ECOLIN_RS22385at 4500.836 kb on + strandat 4500.848 kb on + strandat 4500.893 kb on + strandat 4501.004 kb on - strand, within ECOLIN_RS22390at 4501.030 kb on + strand, within ECOLIN_RS22390at 4501.030 kb on + strand, within ECOLIN_RS22390at 4501.030 kb on + strand, within ECOLIN_RS22390at 4501.031 kb on - strand, within ECOLIN_RS22390at 4501.031 kb on - strand, within ECOLIN_RS22390at 4501.031 kb on - strand, within ECOLIN_RS22390at 4501.031 kb on - strand, within ECOLIN_RS22390at 4501.031 kb on - strand, within ECOLIN_RS22390at 4501.063 kb on + strand, within ECOLIN_RS22390at 4501.270 kb on + strand, within ECOLIN_RS22390at 4501.349 kb on - strand, within ECOLIN_RS22390at 4501.544 kb on - strand, within ECOLIN_RS22390at 4501.570 kb on + strand, within ECOLIN_RS22390at 4501.620 kb on + strand, within ECOLIN_RS22390at 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.724 kb on + strandat 4501.725 kb on - strandat 4501.725 kb on - strandat 4501.725 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas45
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4,499,411 - ECOLIN_RS22375 0.85 -0.1
4,499,411 - ECOLIN_RS22375 0.85 -0.2
4,499,411 - ECOLIN_RS22375 0.85 -0.1
4,499,413 - ECOLIN_RS22375 0.85 -0.6
4,499,413 - ECOLIN_RS22375 0.85 -0.1
4,499,422 + ECOLIN_RS22375 0.87 +0.7
4,499,440 + ECOLIN_RS22375 0.90 -0.0
4,499,440 + ECOLIN_RS22375 0.90 +0.2
4,499,440 + ECOLIN_RS22375 0.90 -0.4
4,499,443 + -0.2
4,499,443 + +0.3
4,499,494 - +0.3
4,499,498 + -0.2
4,499,499 - +0.7
4,499,553 - +0.1
4,499,615 - ECOLIN_RS22380 0.14 -0.4
4,499,615 - ECOLIN_RS22380 0.14 -0.1
4,499,845 + ECOLIN_RS22380 0.40 -0.5
4,500,040 - ECOLIN_RS22380 0.63 +0.0
4,500,096 + ECOLIN_RS22380 0.69 +2.3
4,500,097 - ECOLIN_RS22380 0.69 -0.7
4,500,097 - ECOLIN_RS22380 0.69 +1.0
4,500,108 - ECOLIN_RS22380 0.70 +0.5
4,500,108 - ECOLIN_RS22380 0.70 -0.4
4,500,205 + ECOLIN_RS22380 0.81 -0.3
4,500,217 + ECOLIN_RS22380 0.83 +0.5
4,500,273 + ECOLIN_RS22380 0.89 -0.3
4,500,297 + -0.0
4,500,373 + +0.1
4,500,373 + +0.1
4,500,580 + ECOLIN_RS22385 0.50 +0.2
4,500,581 - ECOLIN_RS22385 0.50 -1.1
4,500,581 - ECOLIN_RS22385 0.50 -0.4
4,500,676 + ECOLIN_RS22385 0.71 -0.4
4,500,678 + ECOLIN_RS22385 0.72 -0.1
4,500,678 + ECOLIN_RS22385 0.72 -0.5
4,500,678 + ECOLIN_RS22385 0.72 -0.7
4,500,678 + ECOLIN_RS22385 0.72 +0.1
4,500,679 - ECOLIN_RS22385 0.72 -0.0
4,500,679 - ECOLIN_RS22385 0.72 -1.1
4,500,679 - ECOLIN_RS22385 0.72 +0.2
4,500,679 - ECOLIN_RS22385 0.72 -1.1
4,500,836 + +0.1
4,500,848 + -0.6
4,500,893 + +0.3
4,501,004 - ECOLIN_RS22390 0.17 +0.7
4,501,030 + ECOLIN_RS22390 0.20 +0.0
4,501,030 + ECOLIN_RS22390 0.20 -0.6
4,501,030 + ECOLIN_RS22390 0.20 +0.1
4,501,031 - ECOLIN_RS22390 0.20 +0.1
4,501,031 - ECOLIN_RS22390 0.20 -0.0
4,501,031 - ECOLIN_RS22390 0.20 -0.4
4,501,031 - ECOLIN_RS22390 0.20 -0.2
4,501,031 - ECOLIN_RS22390 0.20 -0.5
4,501,063 + ECOLIN_RS22390 0.23 +0.1
4,501,270 + ECOLIN_RS22390 0.45 +0.3
4,501,349 - ECOLIN_RS22390 0.54 +0.3
4,501,544 - ECOLIN_RS22390 0.74 +0.1
4,501,570 + ECOLIN_RS22390 0.77 -0.0
4,501,620 + ECOLIN_RS22390 0.82 -0.2
4,501,724 + +0.2
4,501,724 + +0.1
4,501,724 + +0.1
4,501,724 + -0.3
4,501,724 + -0.3
4,501,724 + -0.4
4,501,724 + +1.4
4,501,725 - -0.8
4,501,725 - -0.1
4,501,725 - +1.0

Or see this region's nucleotide sequence