Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS13495

Experiment: Bas45

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS13490 and ECOLIN_RS13495 are separated by 34 nucleotidesECOLIN_RS13495 and ECOLIN_RS13500 are separated by 165 nucleotides ECOLIN_RS13490: ECOLIN_RS13490 - chorismate synthase, at 2,700,249 to 2,701,334 _RS13490 ECOLIN_RS13495: ECOLIN_RS13495 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 2,701,369 to 2,702,301 _RS13495 ECOLIN_RS13500: ECOLIN_RS13500 - endonuclease SmrB, at 2,702,467 to 2,703,018 _RS13500 Position (kb) 2701 2702 2703Strain fitness (log2 ratio) -2 -1 0 1 2at 2701.367 kb on - strandat 2701.502 kb on - strand, within ECOLIN_RS13495at 2701.502 kb on - strand, within ECOLIN_RS13495at 2701.888 kb on - strand, within ECOLIN_RS13495at 2701.888 kb on - strand, within ECOLIN_RS13495at 2701.892 kb on - strand, within ECOLIN_RS13495at 2702.574 kb on + strand, within ECOLIN_RS13500at 2702.874 kb on + strand, within ECOLIN_RS13500at 2703.048 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas45
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2,701,367 - -0.3
2,701,502 - ECOLIN_RS13495 0.14 -0.8
2,701,502 - ECOLIN_RS13495 0.14 +2.1
2,701,888 - ECOLIN_RS13495 0.56 -2.1
2,701,888 - ECOLIN_RS13495 0.56 -0.9
2,701,892 - ECOLIN_RS13495 0.56 -0.7
2,702,574 + ECOLIN_RS13500 0.19 +2.0
2,702,874 + ECOLIN_RS13500 0.74 -0.8
2,703,048 + +0.4

Or see this region's nucleotide sequence