Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12065

Experiment: Bas45

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12060 and ECOLIN_RS12065 are separated by 1 nucleotidesECOLIN_RS12065 and ECOLIN_RS12070 are separated by 55 nucleotides ECOLIN_RS12060: ECOLIN_RS12060 - colanic acid undecaprenyl disphosphate flippase WzxC, at 2,379,299 to 2,380,777 _RS12060 ECOLIN_RS12065: ECOLIN_RS12065 - undecaprenyl-phosphate glucose phosphotransferase, at 2,380,779 to 2,382,173 _RS12065 ECOLIN_RS12070: ECOLIN_RS12070 - colanic acid biosynthesis phosphomannomutase CpsG, at 2,382,229 to 2,383,599 _RS12070 Position (kb) 2380 2381 2382 2383Strain fitness (log2 ratio) -1 0 1at 2380.769 kb on + strandat 2381.547 kb on - strand, within ECOLIN_RS12065at 2381.555 kb on + strand, within ECOLIN_RS12065at 2381.786 kb on + strand, within ECOLIN_RS12065at 2382.231 kb on - strandat 2382.807 kb on - strand, within ECOLIN_RS12070

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas45
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2,380,769 + -0.7
2,381,547 - ECOLIN_RS12065 0.55 -0.0
2,381,555 + ECOLIN_RS12065 0.56 +0.8
2,381,786 + ECOLIN_RS12065 0.72 +0.5
2,382,231 - -1.0
2,382,807 - ECOLIN_RS12070 0.42 +0.4

Or see this region's nucleotide sequence