Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18620

Experiment: Bas44

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18610 and ECOLIN_RS18615 are separated by 8 nucleotidesECOLIN_RS18615 and ECOLIN_RS18620 overlap by 1 nucleotidesECOLIN_RS18620 and ECOLIN_RS18625 are separated by 65 nucleotides ECOLIN_RS18610: ECOLIN_RS18610 - nucleoside triphosphate pyrophosphatase YhdE, at 3,751,329 to 3,751,922 _RS18610 ECOLIN_RS18615: ECOLIN_RS18615 - rod shape-determining protein MreD, at 3,751,931 to 3,752,419 _RS18615 ECOLIN_RS18620: ECOLIN_RS18620 - rod shape-determining protein MreC, at 3,752,419 to 3,753,522 _RS18620 ECOLIN_RS18625: ECOLIN_RS18625 - rod shape-determining protein MreB, at 3,753,588 to 3,754,631 _RS18625 Position (kb) 3752 3753 3754Strain fitness (log2 ratio) -1 0 1at 3751.488 kb on - strand, within ECOLIN_RS18610at 3751.712 kb on + strand, within ECOLIN_RS18610at 3751.793 kb on - strand, within ECOLIN_RS18610at 3753.542 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas44
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3,751,488 - ECOLIN_RS18610 0.27 +0.2
3,751,712 + ECOLIN_RS18610 0.64 -0.2
3,751,793 - ECOLIN_RS18610 0.78 +0.3
3,753,542 - +0.4

Or see this region's nucleotide sequence