Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS15840

Experiment: Bas44

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS15835 and ECOLIN_RS15840 are separated by 175 nucleotidesECOLIN_RS15840 and ECOLIN_RS15845 are separated by 12 nucleotidesECOLIN_RS15845 and ECOLIN_RS15850 overlap by 16 nucleotidesECOLIN_RS15850 and ECOLIN_RS15855 overlap by 4 nucleotides ECOLIN_RS15835: ECOLIN_RS15835 - pitrilysin, at 3,199,870 to 3,202,758 _RS15835 ECOLIN_RS15840: ECOLIN_RS15840 - exodeoxyribonuclease V subunit gamma, at 3,202,934 to 3,206,302 _RS15840 ECOLIN_RS15845: ECOLIN_RS15845 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 3,206,315 to 3,206,638 _RS15845 ECOLIN_RS15850: ECOLIN_RS15850 - DUF2509 family protein, at 3,206,623 to 3,207,030 _RS15850 ECOLIN_RS15855: ECOLIN_RS15855 - prepilin peptidase-dependent protein, at 3,207,027 to 3,207,590 _RS15855 Position (kb) 3202 3203 3204 3205 3206 3207Strain fitness (log2 ratio) -2 -1 0 1at 3202.482 kb on + strandat 3202.483 kb on - strandat 3202.483 kb on - strandat 3202.619 kb on - strandat 3202.700 kb on - strandat 3202.861 kb on + strandat 3202.862 kb on - strandat 3202.862 kb on - strandat 3202.864 kb on - strandat 3202.864 kb on - strandat 3202.896 kb on - strandat 3202.896 kb on - strandat 3203.253 kb on - strandat 3204.111 kb on + strand, within ECOLIN_RS15840at 3204.587 kb on - strand, within ECOLIN_RS15840at 3205.183 kb on + strand, within ECOLIN_RS15840at 3206.316 kb on + strandat 3206.811 kb on - strand, within ECOLIN_RS15850at 3206.811 kb on - strand, within ECOLIN_RS15850at 3206.962 kb on + strand, within ECOLIN_RS15850at 3207.051 kb on - strandat 3207.051 kb on - strandat 3207.053 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas44
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3,202,482 + +1.7
3,202,483 - -0.1
3,202,483 - -0.3
3,202,619 - +0.1
3,202,700 - +0.4
3,202,861 + +0.4
3,202,862 - -0.7
3,202,862 - -0.3
3,202,864 - +0.3
3,202,864 - +0.3
3,202,896 - -0.0
3,202,896 - +0.7
3,203,253 - -1.6
3,204,111 + ECOLIN_RS15840 0.35 -0.7
3,204,587 - ECOLIN_RS15840 0.49 -0.3
3,205,183 + ECOLIN_RS15840 0.67 -2.6
3,206,316 + -0.6
3,206,811 - ECOLIN_RS15850 0.46 +0.0
3,206,811 - ECOLIN_RS15850 0.46 -0.4
3,206,962 + ECOLIN_RS15850 0.83 +0.7
3,207,051 - +0.3
3,207,051 - -0.1
3,207,053 - -0.1

Or see this region's nucleotide sequence