Experiment: Bas44
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS15835 and ECOLIN_RS15840 are separated by 175 nucleotides ECOLIN_RS15840 and ECOLIN_RS15845 are separated by 12 nucleotides ECOLIN_RS15845 and ECOLIN_RS15850 overlap by 16 nucleotides ECOLIN_RS15850 and ECOLIN_RS15855 overlap by 4 nucleotides
ECOLIN_RS15835: ECOLIN_RS15835 - pitrilysin, at 3,199,870 to 3,202,758
_RS15835
ECOLIN_RS15840: ECOLIN_RS15840 - exodeoxyribonuclease V subunit gamma, at 3,202,934 to 3,206,302
_RS15840
ECOLIN_RS15845: ECOLIN_RS15845 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 3,206,315 to 3,206,638
_RS15845
ECOLIN_RS15850: ECOLIN_RS15850 - DUF2509 family protein, at 3,206,623 to 3,207,030
_RS15850
ECOLIN_RS15855: ECOLIN_RS15855 - prepilin peptidase-dependent protein, at 3,207,027 to 3,207,590
_RS15855
Position (kb)
3202
3203
3204
3205
3206
3207 Strain fitness (log2 ratio)
-2
-1
0
1 at 3202.482 kb on + strand at 3202.483 kb on - strand at 3202.483 kb on - strand at 3202.619 kb on - strand at 3202.700 kb on - strand at 3202.861 kb on + strand at 3202.862 kb on - strand at 3202.862 kb on - strand at 3202.864 kb on - strand at 3202.864 kb on - strand at 3202.896 kb on - strand at 3202.896 kb on - strand at 3203.253 kb on - strand at 3204.111 kb on + strand, within ECOLIN_RS15840 at 3204.587 kb on - strand, within ECOLIN_RS15840 at 3205.183 kb on + strand, within ECOLIN_RS15840 at 3206.316 kb on + strand at 3206.811 kb on - strand, within ECOLIN_RS15850 at 3206.811 kb on - strand, within ECOLIN_RS15850 at 3206.962 kb on + strand, within ECOLIN_RS15850 at 3207.051 kb on - strand at 3207.051 kb on - strand at 3207.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas44 remove 3,202,482 + +1.7 3,202,483 - -0.1 3,202,483 - -0.3 3,202,619 - +0.1 3,202,700 - +0.4 3,202,861 + +0.4 3,202,862 - -0.7 3,202,862 - -0.3 3,202,864 - +0.3 3,202,864 - +0.3 3,202,896 - -0.0 3,202,896 - +0.7 3,203,253 - -1.6 3,204,111 + ECOLIN_RS15840 0.35 -0.7 3,204,587 - ECOLIN_RS15840 0.49 -0.3 3,205,183 + ECOLIN_RS15840 0.67 -2.6 3,206,316 + -0.6 3,206,811 - ECOLIN_RS15850 0.46 +0.0 3,206,811 - ECOLIN_RS15850 0.46 -0.4 3,206,962 + ECOLIN_RS15850 0.83 +0.7 3,207,051 - +0.3 3,207,051 - -0.1 3,207,053 - -0.1
Or see this region's nucleotide sequence