Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS32140

Experiment: Bas44

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS30360 and ECOLIN_RS32140 are separated by 216 nucleotidesECOLIN_RS32140 and ECOLIN_RS32255 are separated by 48 nucleotidesECOLIN_RS32255 and ECOLIN_RS01880 are separated by 262 nucleotides ECOLIN_RS30360: ECOLIN_RS30360 - IS1 family transposase, at 375,073 to 375,766 _RS30360 ECOLIN_RS32140: ECOLIN_RS32140 - integrase, at 375,983 to 376,141 _RS32140 ECOLIN_RS32255: ECOLIN_RS32255 - hypothetical protein, at 376,190 to 376,327 _RS32255 ECOLIN_RS01880: ECOLIN_RS01880 - YagU family protein, at 376,590 to 377,204 _RS01880 Position (kb) 375 376 377Strain fitness (log2 ratio) -1 0 1at 375.135 kb on + strandat 375.196 kb on + strand, within ECOLIN_RS30360at 375.643 kb on + strand, within ECOLIN_RS30360at 376.055 kb on + strand, within ECOLIN_RS32140at 376.562 kb on - strandat 376.597 kb on + strandat 376.886 kb on + strand, within ECOLIN_RS01880at 376.957 kb on + strand, within ECOLIN_RS01880

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas44
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375,135 + +0.6
375,196 + ECOLIN_RS30360 0.18 +0.2
375,643 + ECOLIN_RS30360 0.82 +0.3
376,055 + ECOLIN_RS32140 0.45 -1.2
376,562 - -0.3
376,597 + +0.6
376,886 + ECOLIN_RS01880 0.48 +0.4
376,957 + ECOLIN_RS01880 0.60 -0.2

Or see this region's nucleotide sequence