Experiment: Bas44
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS20780 and ECOLIN_RS20785 are separated by 54 nucleotides ECOLIN_RS20785 and ECOLIN_RS20790 are separated by 18 nucleotides ECOLIN_RS20790 and ECOLIN_RS20795 are separated by 6 nucleotides ECOLIN_RS20795 and ECOLIN_RS20800 are separated by 5 nucleotides
ECOLIN_RS20780: ECOLIN_RS20780 - guanylate kinase, at 4,185,763 to 4,186,386
_RS20780
ECOLIN_RS20785: ECOLIN_RS20785 - DNA-directed RNA polymerase subunit omega, at 4,186,441 to 4,186,716
_RS20785
ECOLIN_RS20790: ECOLIN_RS20790 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 4,186,735 to 4,188,843
_RS20790
ECOLIN_RS20795: ECOLIN_RS20795 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 4,188,850 to 4,189,539
_RS20795
ECOLIN_RS20800: ECOLIN_RS20800 - ATP-dependent DNA helicase RecG, at 4,189,545 to 4,191,626
_RS20800
Position (kb) 4186
4187
4188
4189 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4185.749 kb on + strand at 4187.900 kb on - strand, within ECOLIN_RS20790 at 4187.900 kb on - strand, within ECOLIN_RS20790 at 4188.694 kb on - strand at 4188.734 kb on - strand at 4188.819 kb on + strand at 4188.842 kb on - strand at 4189.047 kb on + strand, within ECOLIN_RS20795 at 4189.051 kb on - strand, within ECOLIN_RS20795 at 4189.361 kb on - strand, within ECOLIN_RS20795 at 4189.538 kb on - strand at 4189.562 kb on - strand at 4189.608 kb on - strand at 4189.657 kb on + strand at 4189.712 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas44 remove 4,185,749 + -2.3 4,187,900 - ECOLIN_RS20790 0.55 -0.3 4,187,900 - ECOLIN_RS20790 0.55 -2.9 4,188,694 - -0.9 4,188,734 - +0.3 4,188,819 + -1.6 4,188,842 - -0.3 4,189,047 + ECOLIN_RS20795 0.29 +0.4 4,189,051 - ECOLIN_RS20795 0.29 -0.4 4,189,361 - ECOLIN_RS20795 0.74 -0.8 4,189,538 - -0.3 4,189,562 - -0.1 4,189,608 - -0.2 4,189,657 + -0.5 4,189,712 - -0.8
Or see this region's nucleotide sequence