Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20600

Experiment: Bas44

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20595 and ECOLIN_RS20600 overlap by 1 nucleotidesECOLIN_RS20600 and ECOLIN_RS20605 are separated by 63 nucleotidesECOLIN_RS20605 and ECOLIN_RS20610 are separated by 140 nucleotides ECOLIN_RS20595: ECOLIN_RS20595 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 4,150,160 to 4,151,179 _RS20595 ECOLIN_RS20600: ECOLIN_RS20600 - protein-export chaperone SecB, at 4,151,179 to 4,151,646 _RS20600 ECOLIN_RS20605: ECOLIN_RS20605 - glutaredoxin 3, at 4,151,710 to 4,151,961 _RS20605 ECOLIN_RS20610: ECOLIN_RS20610 - rhodanese-like domain-containing protein, at 4,152,102 to 4,152,533 _RS20610 Position (kb) 4151 4152Strain fitness (log2 ratio) -1 0 1at 4151.675 kb on - strandat 4151.692 kb on - strandat 4151.692 kb on - strandat 4151.692 kb on - strandat 4151.754 kb on - strand, within ECOLIN_RS20605at 4151.966 kb on + strandat 4152.036 kb on - strandat 4152.037 kb on + strandat 4152.037 kb on + strandat 4152.038 kb on - strandat 4152.038 kb on - strandat 4152.044 kb on - strandat 4152.104 kb on - strandat 4152.163 kb on - strand, within ECOLIN_RS20610at 4152.169 kb on - strand, within ECOLIN_RS20610at 4152.234 kb on + strand, within ECOLIN_RS20610at 4152.235 kb on - strand, within ECOLIN_RS20610at 4152.235 kb on - strand, within ECOLIN_RS20610at 4152.235 kb on - strand, within ECOLIN_RS20610at 4152.235 kb on - strand, within ECOLIN_RS20610at 4152.314 kb on + strand, within ECOLIN_RS20610at 4152.315 kb on - strand, within ECOLIN_RS20610at 4152.358 kb on - strand, within ECOLIN_RS20610at 4152.399 kb on - strand, within ECOLIN_RS20610at 4152.443 kb on + strand, within ECOLIN_RS20610at 4152.450 kb on - strand, within ECOLIN_RS20610at 4152.556 kb on - strandat 4152.582 kb on + strandat 4152.584 kb on + strandat 4152.605 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas44
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4,151,675 - -0.2
4,151,692 - +0.2
4,151,692 - +0.6
4,151,692 - +0.1
4,151,754 - ECOLIN_RS20605 0.17 -0.2
4,151,966 + +1.6
4,152,036 - +0.1
4,152,037 + -0.1
4,152,037 + +0.2
4,152,038 - +0.1
4,152,038 - -0.2
4,152,044 - +0.2
4,152,104 - +0.3
4,152,163 - ECOLIN_RS20610 0.14 -0.3
4,152,169 - ECOLIN_RS20610 0.16 +0.9
4,152,234 + ECOLIN_RS20610 0.31 -0.4
4,152,235 - ECOLIN_RS20610 0.31 +0.1
4,152,235 - ECOLIN_RS20610 0.31 -0.5
4,152,235 - ECOLIN_RS20610 0.31 -0.5
4,152,235 - ECOLIN_RS20610 0.31 +0.1
4,152,314 + ECOLIN_RS20610 0.49 -0.1
4,152,315 - ECOLIN_RS20610 0.49 +0.9
4,152,358 - ECOLIN_RS20610 0.59 -0.2
4,152,399 - ECOLIN_RS20610 0.69 +0.1
4,152,443 + ECOLIN_RS20610 0.79 -0.5
4,152,450 - ECOLIN_RS20610 0.81 -0.2
4,152,556 - +0.2
4,152,582 + +0.4
4,152,584 + -0.1
4,152,605 - +0.1

Or see this region's nucleotide sequence