Experiment: Bas44
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS00110 and ECOLIN_RS00115 are separated by 95 nucleotides ECOLIN_RS00115 and ECOLIN_RS00120 are separated by 102 nucleotides ECOLIN_RS00120 and ECOLIN_RS00125 are separated by 7 nucleotides ECOLIN_RS00125 and ECOLIN_RS00130 are separated by 42 nucleotides
ECOLIN_RS00110: ECOLIN_RS00110 - transcriptional activator NhaR, at 21,389 to 22,294
_RS00110
ECOLIN_RS00115: ECOLIN_RS00115 - 30S ribosomal protein S20, at 22,390 to 22,653
_RS00115
ECOLIN_RS00120: ECOLIN_RS00120 - DUF2575 family protein, at 22,756 to 22,974
_RS00120
ECOLIN_RS00125: ECOLIN_RS00125 - bifunctional riboflavin kinase/FAD synthetase, at 22,982 to 23,923
_RS00125
ECOLIN_RS00130: ECOLIN_RS00130 - isoleucine--tRNA ligase, at 23,966 to 26,782
_RS00130
Position (kb)
22
23 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.765 kb on + strand, within ECOLIN_RS00110 at 21.817 kb on + strand, within ECOLIN_RS00110 at 22.127 kb on + strand, within ECOLIN_RS00110 at 22.246 kb on + strand at 22.247 kb on - strand at 22.247 kb on - strand at 22.255 kb on + strand at 22.776 kb on + strand at 22.777 kb on - strand at 22.777 kb on - strand at 22.940 kb on + strand, within ECOLIN_RS00120 at 22.941 kb on - strand, within ECOLIN_RS00120 at 22.947 kb on + strand, within ECOLIN_RS00120 at 22.949 kb on + strand, within ECOLIN_RS00120 at 23.930 kb on + strand at 23.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas44 remove 21,765 + ECOLIN_RS00110 0.42 -0.8 21,817 + ECOLIN_RS00110 0.47 -0.1 22,127 + ECOLIN_RS00110 0.81 +0.4 22,246 + +0.6 22,247 - -0.5 22,247 - -1.6 22,255 + -0.2 22,776 + +0.5 22,777 - -1.7 22,777 - -2.1 22,940 + ECOLIN_RS00120 0.84 -0.0 22,941 - ECOLIN_RS00120 0.84 -0.4 22,947 + ECOLIN_RS00120 0.87 -0.3 22,949 + ECOLIN_RS00120 0.88 -0.8 23,930 + -1.6 23,930 + -2.2
Or see this region's nucleotide sequence