Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03325

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03320 and ECOLIN_RS03325 are separated by 170 nucleotidesECOLIN_RS03325 and ECOLIN_RS03330 are separated by 120 nucleotidesECOLIN_RS03330 and ECOLIN_RS03335 are separated by 356 nucleotides ECOLIN_RS03320: ECOLIN_RS03320 - alkyl hydroperoxide reductase subunit C, at 686,159 to 686,722 _RS03320 ECOLIN_RS03325: ECOLIN_RS03325 - alkyl hydroperoxide reductase subunit F, at 686,893 to 688,458 _RS03325 ECOLIN_RS03330: ECOLIN_RS03330 - universal stress protein UspG, at 688,579 to 689,007 _RS03330 ECOLIN_RS03335: ECOLIN_RS03335 - nucleoside diphosphate kinase regulator, at 689,364 to 689,774 _RS03335 Position (kb) 686 687 688 689Strain fitness (log2 ratio) -1 0 1at 685.896 kb on + strandat 685.958 kb on + strandat 686.154 kb on + strandat 686.156 kb on + strandat 686.295 kb on + strand, within ECOLIN_RS03320at 686.374 kb on + strand, within ECOLIN_RS03320at 686.631 kb on + strand, within ECOLIN_RS03320at 687.536 kb on + strand, within ECOLIN_RS03325at 687.536 kb on + strand, within ECOLIN_RS03325at 687.536 kb on + strand, within ECOLIN_RS03325at 687.730 kb on + strand, within ECOLIN_RS03325at 688.091 kb on + strand, within ECOLIN_RS03325at 688.166 kb on + strand, within ECOLIN_RS03325at 689.066 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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685,896 + +0.4
685,958 + -0.2
686,154 + -0.7
686,156 + +0.5
686,295 + ECOLIN_RS03320 0.24 -0.1
686,374 + ECOLIN_RS03320 0.38 -0.3
686,631 + ECOLIN_RS03320 0.84 -0.1
687,536 + ECOLIN_RS03325 0.41 -0.1
687,536 + ECOLIN_RS03325 0.41 -1.2
687,536 + ECOLIN_RS03325 0.41 -0.0
687,730 + ECOLIN_RS03325 0.53 +0.1
688,091 + ECOLIN_RS03325 0.77 -0.5
688,166 + ECOLIN_RS03325 0.81 +0.1
689,066 + -0.1

Or see this region's nucleotide sequence