Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02650

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02645 and ECOLIN_RS02650 are separated by 150 nucleotidesECOLIN_RS02650 and ECOLIN_RS02655 are separated by 105 nucleotidesECOLIN_RS02655 and ECOLIN_RS02660 are separated by 51 nucleotides ECOLIN_RS02645: ECOLIN_RS02645 - peptidylprolyl isomerase, at 541,220 to 543,091 _RS02645 ECOLIN_RS02650: ECOLIN_RS02650 - helix-hairpin-helix domain-containing protein, at 543,242 to 543,613 _RS02650 ECOLIN_RS02655: ECOLIN_RS02655 - long-chain acyl-CoA thioesterase FadM, at 543,719 to 544,117 _RS02655 ECOLIN_RS02660: ECOLIN_RS02660 - 7-cyano-7-deazaguanine synthase QueC, at 544,169 to 544,864 _RS02660 Position (kb) 543 544Strain fitness (log2 ratio) -1 0 1at 543.549 kb on + strand, within ECOLIN_RS02650at 543.679 kb on + strandat 543.694 kb on - strandat 543.988 kb on + strand, within ECOLIN_RS02655at 544.042 kb on + strand, within ECOLIN_RS02655at 544.531 kb on - strand, within ECOLIN_RS02660

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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543,549 + ECOLIN_RS02650 0.83 -0.4
543,679 + +0.4
543,694 - -0.1
543,988 + ECOLIN_RS02655 0.67 -0.1
544,042 + ECOLIN_RS02655 0.81 +0.3
544,531 - ECOLIN_RS02660 0.52 -0.7

Or see this region's nucleotide sequence