Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12710

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12705 and ECOLIN_RS12710 are separated by 117 nucleotidesECOLIN_RS12710 and ECOLIN_RS12715 are separated by 38 nucleotides ECOLIN_RS12705: ECOLIN_RS12705 - mannitol dehydrogenase family protein, at 2,528,835 to 2,530,301 _RS12705 ECOLIN_RS12710: ECOLIN_RS12710 - zinc-binding GTPase YeiR, at 2,530,419 to 2,531,405 _RS12710 ECOLIN_RS12715: ECOLIN_RS12715 - Kdo(2)-lipid A phosphotransferase, at 2,531,444 to 2,532,157 _RS12715 Position (kb) 2530 2531 2532Strain fitness (log2 ratio) -1 0 1at 2529.995 kb on - strand, within ECOLIN_RS12705at 2530.315 kb on + strandat 2532.294 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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2,529,995 - ECOLIN_RS12705 0.79 +0.2
2,530,315 + -0.4
2,532,294 - -0.4

Or see this region's nucleotide sequence