Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS11490

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS11485 and ECOLIN_RS11490 are separated by 100 nucleotidesECOLIN_RS11490 and ECOLIN_RS11495 are separated by 161 nucleotides ECOLIN_RS11485: ECOLIN_RS11485 - toxic metabolite efflux MATE transporter YeeO, at 2,236,422 to 2,237,909 _RS11485 ECOLIN_RS11490: ECOLIN_RS11490 - tRNA-Asn, at 2,238,010 to 2,238,085 _RS11490 ECOLIN_RS11495: ECOLIN_RS11495 - integrase arm-type DNA-binding domain-containing protein, at 2,238,247 to 2,239,518 _RS11495 Position (kb) 2238 2239Strain fitness (log2 ratio) -1 0 1at 2237.124 kb on + strand, within ECOLIN_RS11485at 2237.279 kb on + strand, within ECOLIN_RS11485at 2237.726 kb on - strand, within ECOLIN_RS11485at 2238.175 kb on + strandat 2238.221 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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2,237,124 + ECOLIN_RS11485 0.47 -0.0
2,237,279 + ECOLIN_RS11485 0.58 -0.2
2,237,726 - ECOLIN_RS11485 0.88 +0.6
2,238,175 + +0.2
2,238,221 + -0.1

Or see this region's nucleotide sequence