Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20000

Experiment: Bas22

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS19995 and ECOLIN_RS20000 are separated by 53 nucleotidesECOLIN_RS20000 and ECOLIN_RS20010 are separated by 334 nucleotides ECOLIN_RS19995: ECOLIN_RS19995 - glutathione-disulfide reductase, at 4,013,590 to 4,014,942 _RS19995 ECOLIN_RS20000: ECOLIN_RS20000 - damage-inducible type I toxin DinQ, at 4,014,996 to 4,015,079 _RS20000 ECOLIN_RS20010: ECOLIN_RS20010 - glutaredoxin-dependent arsenate reductase, at 4,015,414 to 4,015,839 _RS20010 Position (kb) 4014 4015 4016Strain fitness (log2 ratio) -1 0 1at 4014.119 kb on - strand, within ECOLIN_RS19995at 4014.480 kb on + strand, within ECOLIN_RS19995at 4014.702 kb on + strand, within ECOLIN_RS19995at 4014.961 kb on + strandat 4014.961 kb on + strandat 4014.979 kb on + strandat 4015.045 kb on - strand, within ECOLIN_RS20000at 4015.156 kb on + strandat 4015.167 kb on + strandat 4015.167 kb on + strandat 4015.291 kb on + strandat 4015.292 kb on - strandat 4015.304 kb on - strandat 4015.309 kb on + strandat 4015.309 kb on + strandat 4015.309 kb on + strandat 4015.310 kb on - strandat 4015.310 kb on - strandat 4015.310 kb on - strandat 4015.314 kb on - strandat 4015.314 kb on - strandat 4015.328 kb on - strandat 4015.359 kb on - strandat 4015.359 kb on - strandat 4015.461 kb on + strand, within ECOLIN_RS20010at 4015.462 kb on - strand, within ECOLIN_RS20010at 4015.462 kb on - strand, within ECOLIN_RS20010at 4015.464 kb on + strand, within ECOLIN_RS20010at 4015.507 kb on - strand, within ECOLIN_RS20010at 4015.510 kb on + strand, within ECOLIN_RS20010at 4015.589 kb on + strand, within ECOLIN_RS20010at 4015.656 kb on - strand, within ECOLIN_RS20010at 4015.666 kb on - strand, within ECOLIN_RS20010at 4015.837 kb on + strandat 4015.847 kb on - strandat 4015.849 kb on - strandat 4016.050 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas22
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4,014,119 - ECOLIN_RS19995 0.39 -0.0
4,014,480 + ECOLIN_RS19995 0.66 -0.9
4,014,702 + ECOLIN_RS19995 0.82 -0.6
4,014,961 + +0.1
4,014,961 + +0.2
4,014,979 + -0.2
4,015,045 - ECOLIN_RS20000 0.58 +0.3
4,015,156 + -0.3
4,015,167 + -0.1
4,015,167 + -0.6
4,015,291 + -0.2
4,015,292 - -0.1
4,015,304 - +0.7
4,015,309 + +0.4
4,015,309 + -0.2
4,015,309 + +0.6
4,015,310 - -0.3
4,015,310 - -0.3
4,015,310 - -0.0
4,015,314 - +1.6
4,015,314 - -0.2
4,015,328 - -0.1
4,015,359 - +0.1
4,015,359 - +0.2
4,015,461 + ECOLIN_RS20010 0.11 -0.4
4,015,462 - ECOLIN_RS20010 0.11 -0.2
4,015,462 - ECOLIN_RS20010 0.11 -0.0
4,015,464 + ECOLIN_RS20010 0.12 +0.1
4,015,507 - ECOLIN_RS20010 0.22 -0.0
4,015,510 + ECOLIN_RS20010 0.23 -0.6
4,015,589 + ECOLIN_RS20010 0.41 -0.4
4,015,656 - ECOLIN_RS20010 0.57 +0.2
4,015,666 - ECOLIN_RS20010 0.59 +0.2
4,015,837 + +1.7
4,015,847 - -0.2
4,015,849 - -0.4
4,016,050 - +0.4

Or see this region's nucleotide sequence