Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12950

Experiment: Bas22

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12945 and ECOLIN_RS12950 are separated by 16 nucleotidesECOLIN_RS12950 and ECOLIN_RS12955 are separated by 85 nucleotides ECOLIN_RS12945: ECOLIN_RS12945 - phosphotransferase RcsD, at 2,572,510 to 2,575,182 _RS12945 ECOLIN_RS12950: ECOLIN_RS12950 - response regulator transcription factor RcsB, at 2,575,199 to 2,575,849 _RS12950 ECOLIN_RS12955: ECOLIN_RS12955 - two-component system sensor histidine kinase RcsC, at 2,575,935 to 2,578,784 _RS12955 Position (kb) 2575 2576Strain fitness (log2 ratio) -1 0 1at 2574.733 kb on - strand, within ECOLIN_RS12945at 2574.848 kb on + strand, within ECOLIN_RS12945at 2574.854 kb on + strand, within ECOLIN_RS12945at 2575.180 kb on + strandat 2576.154 kb on - strandat 2576.394 kb on - strand, within ECOLIN_RS12955

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas22
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2,574,733 - ECOLIN_RS12945 0.83 -0.4
2,574,848 + ECOLIN_RS12945 0.87 -0.3
2,574,854 + ECOLIN_RS12945 0.88 -0.4
2,575,180 + +0.1
2,576,154 - +0.5
2,576,394 - ECOLIN_RS12955 0.16 +0.7

Or see this region's nucleotide sequence